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  7. 17130532

MHC Class II Monoclonal Antibody (HIS19), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0920-95

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Product Code. 17130532

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Description

Description

Description: The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B. This antibody reacts with all rat haplotypes except the RT1n (including BN and MAXX strains). The rat MHC class II (I-A equivalent), RT1B, is expressed by antigen presenting cells including B cells, dendritic cells and macrophages. Applications Reported: The HIS19 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. Applications Tested: The HIS19 antibody has been tested by flow cytometric analysis of rat spleen or lymph node cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

MHC Class II Monoclonal antibody specifically detects MHC Class II in Rat samples. It is validated for Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
TRUSTED_SUSTAINABILITY
Specifications

Specifications

MHC Class II
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Mouse
10 mg
Primary
4° C
Liquid
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
HIS19
Unconjugated
B-L beta minor; B-LB; BLB major; BLB1; BLB2; B-LB2; B-LB21; Bl-Beta II protein; FECA-DRB; GSP-BLB1; major histocompatibility complex class II B; Major histocompatibility complex class II beta chain BLB2, (similar to HLA class II, D beta chain); MHC 2; MHC Class II; MHC class II antigen beta chain; MHC class II antigen DRB; MHC class II beta 1 domain; MHC class II beta chain 1; MHC class II beta chain 2; MHC class II B-L beta chain; MHC II; MHCCII; MHCFECA-DRB
Affinity chromatography
RUO
Rat
Antibody
IgG1
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