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Invitrogen™ Human MBL Oligomer ELISA Kit

Product Code. 17883568
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96 Tests
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96 tests
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Product Code. 17883568 Supplier Invitrogen™ Supplier No. BMS23292
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Description

ELISA

The Human Mannose Binding Lectin (Hu MBL-2) ELISA quantitates Hu MBL-2 in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu MBL-2. Principle of the method The Human MBL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MBL2 is a calcium-dependent lectin involved in innate immune defense. It binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. It also binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. MBL2 may bind DNA.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P11226
Assay Range 0.05-40 ng/mL
Assay Sensitivity 102 pg/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Protein Family Lectin
Sample Type Plasma, Serum
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias 27 kDa mannan-binding protein monomeric subunit; Ab2-001; Ab2-011; cMBl; COLEC1; collectin; Collectin1; collectin-1; c-type lectin; HSMBPC; L-MBP; mannan-binding lectin; mannan-binding lectin C; mannan-binding protein; mannose binding lectin (C); mannose binding lectin 2; mannose binding lectin 2 (protein C); mannose binding lectin, liver (A); mannose binding lectin, liver (C); mannose-binding lectin; mannose-binding lectin (protein C) 2; mannose-binding lectin (protein C) 2, soluble; mannose-binding lectin (protein C) 2, soluble (opsonic defect); mannose-binding lectin 2, soluble (opsonic defect); mannose-binding lectin C; mannose-binding lectin precursor protein; mannose-binding lectin-C; mannose-binding protein; Mannose-binding protein C; mannose-binding protein C (liver); mbl; MBL2; MBL2D; MBL-C; MBP; MBP1; MBPC; MBP-C; MBPD; MGC116832; MGC116833; pMBP-27; RA-reactive factor P28A subunit; ra-reactive factor polysaccharide-binding component p28A; RaRF p28A; RARF/P28A; serum protein; MBL; soluble mannose-binding lectin27 kDa mannan-binding protein monomeric subunit; Ab2-001; Ab2-011; cMBl; COLEC1; collectin; Collectin1; collectin-1; c-type lectin; HSMBPC; L-MBP; mannan-binding lectin; mannan-binding lectin C; mannan-binding protein; mannose binding lectin (C); mannose binding lectin 2; mannose binding lectin 2 (protein C); mannose binding lectin, liver (A); mannose binding lectin, liver (C); mannose-binding lectin; mannose-binding lectin (protein C) 2; mannose-binding lectin (protein C) 2, soluble; mannose-binding lectin (protein C) 2, soluble (opsonic defect); mannose-binding lectin 2, soluble (opsonic defect); mannose-binding lectin C; mannose-binding lectin precursor protein; mannose-binding lectin-C; mannose-binding protein; Mannose-binding protein C; mannose-binding protein C (liver); mbl; MBL2; MBL2D; MBL-C; MBP; MBP1; MBPC; MBP-C; MBPD; MGC116832; MGC116833; pMBP-27; RA-reactive factor P28A subunit; ra-reactive factor polysaccharide-binding component p28A; RaRF p28A; RARF/P28A; serum protein; MBL; soluble mannose-binding lectin; COLEC1; HSMBPC; MBL; MBL2; MBL2D; MBP; MBP-C; MBP1; MBPD
Gene ID (Entrez) 4153
Gene Symbol MBL2
Interassay CV <10%
Intraassay CV <6%
Kit Contents Pre-coated 96 well plate, Standard (2 vials), HRP Detection Antibody, Assay buffer Concentrate, Wash buffer concentrate, TMB, Stop solution, Adhesive Plate Covers
Label or Dye HRP
Protein Aliases Collectin-1, mannan-binding lectin, Mannan-binding protein, Mannose-binding lectin, mannose-binding lectin (protein C) 2, soluble (opsonic defect), mannose-binding lectin 2, Mannose-binding protein C, MBL2, MBP-C, MBP1
Quantity 96 Tests
Regulatory Status RUO
Shipping Condition Wet Ice
Storage Requirements 2-8°C
Target MBL2
Test Time 1 hr 20 min
Total Assay Time 3 hr 15 min
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