missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Immunohistochemistry Kits and Reagents
  5. 15333806

Thermo Scientific™ LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, rabbit

Product Code. 15333806
Change view
Click to view available options
Quantity:
800 x 20 μL assays
Unit Size:
1 set
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
15333806 800 x 20 μL assays 1 set
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 15333806 Supplier Thermo Scientific™ Supplier No. A15142

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

The LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR).

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4839:

The LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR). The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescent small-molecule PXR ligand (Fluormone™ PXR (SXR) Green), and a human PXR (SXR) ligand-binding domain that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:

When running the LanthaScreen™ TR-FRET PXR (SXR) competitive binding assay, a Tb-labeled anti-GST antibody is used to indirectly label the receptor by binding to its GST tag. Competitive ligand binding is detected by a test compound's ability to displace a fluorescent ligand (tracer) from the receptor, which results in a loss of FRET signal between the Tb-anti-GST antibody and the tracer (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, 'addition-only' format.

TRUSTED_SUSTAINABILITY

Specifications

Color Green
Conjugate Tb (Terbium)
Description LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, Rabbit, 800 x 20μL Assays, Green, 384-Well Plate Format, Fluorescent Detection Method, Nuclear Receptors Druggable Target, Tb (Terbium) Label, TR-FRET Readout, Nuclear Receptor Assay
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
Includes Tb-anti-GST Antibody (Rabbit), PXR(SXR)-LBD, GST, fluormone PXR(SXR) green, TR-FRET PXR(SXR) assay buffer, 1M DTT
Ligand Coupling Method Competitive ligand binding
Quantity 800 x 20 μL assays
Regulatory Status For research use only. Not for use in diagnostic procedures
Packaging 384-well Plate
Storage Buffer TR-FRET PXR(SXR) assay buffer
Content And Storage • Tb-anti-GST Antibody (Rabbit) (store at -20°C)
• PXR(SXR)-LBD, GST (-80°C)
• Fluormone PXR(SXR) Green (-20°C)
• TR-FRET PXR(SXR) Assay Buffer (4°C)
• 1M DTT (-20°C or -80°C)
Assay Category Biochemical Assay
Druggable Target Nuclear Receptors
Readout TR-FRET (Binding)
Product Line LanthaScreen
For Use With (Application) Nuclear Receptor Assay, TR-FRET
Ligand PXR
Product Type TR-FRET PXR (SXR) Competitive Binding Assay Kit
No. of Assays 800 x 20 μL
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.