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Thermo Scientific™ KingFisher™ PlasmidPro Maxi Processor Endotoxin-Free Cartridge

Product Code. 30131894 Shop All Thermo Scientific Products
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Product Code. 30131894 Supplier Thermo Scientific™ Supplier No. A54072

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Description

The KingFisher™ PlasmidPro Maxi Processor Endotoxin-Free Cartridge is used with the KingFisher™ PlasmidPro Maxi Processor for fully automated isolation of endotoxin-free plasmid DNA in under 75 minutes.

The KingFisher™ PlasmidPro Maxi Processor Endotoxin-Free Cartridge is used with the KingFisher™ PlasmidPro Maxi Processor for fully automated isolation of endotoxin-free plasmid DNA in under 75 minutes. Purify high-quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines or genome engineering.

Features and benefits

  • Convenient one-piece cartridge pre-filled with all necessary reagents
  • Collection tube included for simple removal and storage of plasmid DNA
  • Designed for mess-free clean-up and waste disposal
  • Plasmid DNA contains less than <0.1 EU/μg

Additional product details

The KingFisher™ PlasmidPro Maxi Processor is a cutting-edge solution designed for fully automated maxi-scale plasmid purification.

At the end of the procedure, the system delivers endotoxin-free plasmid DNA. The purified DNA is resuspended in TE buffer, ensuring optimal quality and compatibility with downstream applications.

TRUSTED_SUSTAINABILITY

Specifications

Product Type Cartridge
Content And Storage 4 KingFisher™ PlasmidPro Maxi Processor Endotoxin-free Cartridges

Store at room temperature (15°C to 25°C).
For Use With (Application) Plasmid purification
For Use With (Equipment) KingFisher™ PlasmidPro Maxi Processor
Purification Target Plasmid DNA
Quantity 4 Preps
Run Time <75 min.
Sample Volume 150 mL
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Can I resuspend a frozen pellet for use with the KingFisher PlasmidPro Maxi Processor?

When using frozen pellets as a starting point, please consider the following guidelines:

  • The pellet should represent 150 mL of the specified culture.
  • Fully resuspend the pellet in 150 mL of 1X PBS or the parent media.
  • The resuspension can be achieved by either pipetting up and down or vigorous vortexing.
What are the storage conditions for the KingFisher PlasmidPro Maxi Processor Cartridge? Is refrigeration required?

We recommend storing the KingFisher PlasmidPro Maxi Processor Cartridge at room temperature. Refrigeration is not needed.
Can I reuse the KingFisher PlasmidPro Maxi Processor Cartridge?

The KingFisher PlasmidPro Maxi Processor Cartridge is single-use and should be disposed of after use, minimizing clean-up and risk of cross-contamination. Re-use of the cartridge will contaminate and potentially harm the instrument.
Is the KingFisher PlasmidPro Maxi Processor Cartridge animal origin-free?

No. The RNaseA used in the KingFisher PlasmidPro Maxi Processor Cartridge is not animal origin-free. Review the Certificate of Analysis (COA) for more details.

What solution is the plasmid DNA sample resuspended in at the end of the purification using the KingFisher PlasmidPro Maxi Processor?

At the end of the purification using the KingFisher PlasmidPro Maxi Processor, the plasmid DNA sample is resuspended in TE buffer (10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA).
What run data is generated and/or captured by the KingFisher PlasmidPro Maxi Processor?

The KingFisher PlasmidPro Maxi Processor is designed to automatically capture the following information for every run:

  • Cartridge Lot Number
  • Cartridge Expiration Date
  • Sample Name
  • Sample Run Start Time
  • Sample Run Finish Time
  • Run Errors (Type/Details)

By automatically reading the barcodes, the KingFisher PlasmidPro Maxi Processor helps ensure accurate and efficient tracking of important information related to the cartridge, samples, and run details.
Is there any required downtime between runs on the KingFisher PlasmidPro Maxi Processor?

No. The KingFisher PlasmidPro Maxi Processor does not require any downtime between runs. The system does not employ any mechanical, optical, or electrical components that have a “wait” time to reset between purification runs. As soon as the used cartridge is removed from the instrument, a new cartridge can be inserted and run immediately.
What is the total run-time for the purification process using the KingFisher PlasmidPro Maxi Processor?

The run time for the purification process is 73 min.
What are the guidelines for growing E. coli for optimal yield and purity results when using the KingFisher PlasmidPro Maxi Processor, e.g., OD range, growth media, plasmid size?

  • Use overnight Luria-Bertani medium optimally grown for at least 12 hr but not greater than 16 hr. Grow sufficient culture volume to support 150 mL.
  • Use bacterial cultures with a measured cell density of approximately 1.5 - 6.0 absorbance at 600 nm (A600).
  • Use a high-copy or low-copy plasmid between 3 and 10 kb cultured in Luria-Bertani medium to obtain maximal yield and purity of plasmid DNA.

Note: Cell density or plasmid kb outside of the recommendation will result in reduced yield and purity.
How does the KingFisher PlasmidPro Maxi Processor work?

The KingFisher PlasmidPro Maxi Processor is designed for fully automated, maxi-scale plasmid purification from cultures grown in Luria-Bertani medium or Terrific Broth. The instrument utilizes a self-contained cartridge pre-filled with the necessary reagents to process the culture by filtration and alkaline lysis, to minimize handling and preparation time. It uses a system of pumps and valves to move the sample and reagents in the cartridge to perform all the steps of the purification process in under 75 mins and provides endotoxin-free plasmid DNA resuspended in TE buffer, at the end of the procedure.

For Research Use Only. Not for use in diagnostic procedures.

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