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  7. Invitrogen™ Platinum™ GenoType Tsp DNA Polymerase 

Invitrogen™ Platinum™ GenoType Tsp DNA Polymerase

Product Code. 10524443
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10524443 400 Reactions Each
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To receive the discount customers must purchase 2 of the same product at list price in a single order to receive 30% discount. There is no limit to the multiples of 2 that customers can buy. Use promo code ”27974” to get your promotional price
Product Code. 10524443 Supplier Invitrogen™ Supplier No. 11448024

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497.00 EUR valid until 2026-05-15
BEST PRICE on Promo! Use promo code "27974" to get your promotional price.
This item is not returnable. View return policy
To receive the discount customers must purchase 2 of the same product at list price in a single order to receive 30% discount. There is no limit to the multiples of 2 that customers can buy. Use promo code ”27974” to get your promotional price

Description

Includes

Platinum GenoType TspDNA Polymerase is supplied with 10X PCR Buffer (200mM Tris-HCl (pH 8.4), 500mM KCl), and a vial of 50mM MgCl2.

Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications.

Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications. The enzyme is pre-complexed with a thermolabile inhibitor containing monoclonal antibodies to Tsp DNA polymerase, providing an automatic hot-start method that improves PCR specificity and allows for room temperature reaction assembly.

Platinum GenoType Tsp DNA Polymerase features

  • Minimal non-templated nucleotide addition to PCR products
  • Lacks both 5' and 3' exonuclease activity
  • Amplifies fragments up to 500 bp
  • Room temperature reaction assembly

Application

Amplification of dinucleotide repeat loci in applications in which elimination of non-templated nucleotide addition is desirable.

Unit definition

One unit of Platinum GenoType Tsp DNA Polymerase has been functionally determined to be equivalent to one unit of Taq DNA Polymerase in amplification of dinucleotide repeats using standard Taq DNA polymerase reaction conditions. One Tsp DNA polymerase unit approximates 2.5 activity units. An activity unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min. at 74°C under optimized reaction conditions.

Order Info

Approved for shipment on Wet or Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage • Platinum GenoType Tsp DNA Polymerase (50 μL at 5 Tsp U/μL)
• 10X PCR Buffer (1.25 mL)
• 50 mM MgCl2 (1 mL)

Store at -20°C. Guaranteed stable for 6 months when properly stored.
GC-Rich PCR Performance Low
Polymerase Tsp Genotyping Polymerase
Reaction Speed Standard
Quantity 250 units
Shipping Condition Wet or Dry Ice
For Use With (Application) Hot-start PCR
Fidelity (vs. Taq) 1X
Hot Start Built-In Hot Start
No. of Reactions 400 Reactions
Overhang Blunt
Reaction Format Separate Components
Size (Final Product) 500 bp or less
Volume 50 μL
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For Research Use Only. Not for use in diagnostic procedures.

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