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Invitrogen™ pBAD/Myc-His Kit

Product Code. 10580333
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Product Code. 10580333 Supplier Invitrogen™ Supplier No. V44001

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Description

Includes

20μg of pBAD/Myc-His A, B and C vector, 20μg of pBAD/Myc-His/lacZ, 20% L-arabinose, LMG194 and TOP10 E. coli stabs.

Expresses native proteins or fusion proteins with a C-terminal tag in a tightly regulated fashion

  • araBAD promoter for tightly regulated expression
  • Translation initiation signals optimized for E. coli expression
  • C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin or detection with Anti-His(C-term) Antibody
  • C-terminal c-myc epitope for detection and analysis with Anti-myc Antibody
  • Three vectors are provided (A, B, and C): Each has C-terminal tag in different reading frame relative to multiple cloning site to simplify in-frame cloning of gene

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Inducible
Inducing Agent Arabinose
Promoter araBAD
Product Type Bacterial Expression Vector
Selection Agent (Eukaryotic) None
Content And Storage The pBAD/Myc-His Kit includes 20 μg of pBAD/Myc-His A, B, & C vector, 20 μg of pBAD/Myc-His/lacZ, 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR)
Cleavage EK (Enterokinase) Recognition Site
Protein Tag c-Myc Epitope Tag
Cloning Method Restriction Enzyme/MCS
Quantity 1 kit
Vector pBAD
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For Research Use Only. Not for use in diagnostic procedures.

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