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Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli

Supplied at transformation efficiency of 1 x 109 cfu/ μg supercoiled DNA that's ideal for high-efficiency cloning and plasmid propagation

Brand:  Invitrogen™ C404052

397.60 EUR valid until 2022-10-31
Use promo code "16498" to get your promotional price.



Additional Details : Weight : 0.02000kg

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Product Code. 10082692

  • 522.00 EUR / 20 reactions
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Description

Description

Provides stable replication of high copy number plasmids. The genotype of TOP10 cells is similar to that of DH10B™ strain.

  • hsdR for efficient transformation of unmethylated DNA from PCR amplifications
  • mcrA for efficient transformation of methylated DNA from genomic preparations
  • lacZΔM15 for blue/white color screening of recombinant clones
  • endA1 for cleaner preparations of DNA—get better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
  • recA1 for reduced occurrence of nonspecific recombination in cloned DNA
  • Bacterial/Yeast Strain: TOP10

Cloning, Transformation

Specifications

Specifications

One Shot
One Shot™
20 Reactions
Electrocompetent
Yes
Yes
Yes
May be used for plasmids > 20 kb
Dry Ice
E. coli
High Efficiency (> 10^9 cfu⁄µg)
Not High-throughput Compatible (Manual)
Transformation, Cloning
E. coil
Contains:
• One Shot™ TOP10 Electrocomp™ E. coli: 21 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
Not Suitable for cloning unstable DNA
Lacks F' Episome
TOP10
Not for ccdB vector propagation
Not Suitable for preparing unmethylated DNA
Yes
No
> 1x10^10
Videos
SDS
Documents
Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.