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Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli

Supplied at transformation efficiency of 1 x 109 cfu/ μg supercoiled DNA that's ideal for high-efficiency cloning and plasmid propagation

Brand:  Invitrogen™ C404052

458.85 EUR valid until 2021-10-30
Use promo code "14806" to get your promotional price.



Additional Details : Weight : 0.02000kg

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Product Code. 10082692

  • 483.00 EUR / 20 reactions

In stock, ready to ship: 6

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Description

Description

Provides stable replication of high copy number plasmids. The genotype of TOP10 cells is similar to that of DH10B™ strain.

  • hsdR for efficient transformation of unmethylated DNA from PCR amplifications
  • mcrA for efficient transformation of methylated DNA from genomic preparations
  • lacZΔM15 for blue/white color screening of recombinant clones
  • endA1 for cleaner preparations of DNA—get better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
  • recA1 for reduced occurrence of nonspecific recombination in cloned DNA
  • Bacterial/Yeast Strain: TOP10

Cloning, Transformation

Specifications

Specifications

TOP10
Yes
Electrocompetent
One Shot
Yes
Not Suitable for preparing unmethylated DNA
Not for ccdB vector propagation
Yes
E. coli
High Efficiency (> 10^9 cfu⁄µg)
Transformation, Cloning
Yes
Not Suitable for cloning unstable DNA
Lacks F' Episome
Not High-throughput Compatible (Manual)
May be used for plasmids > 20 kb
One Shot™
20 reactions
Dry Ice
No
> 1x10^10
E. coil
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For Research Use Only. Not for use in diagnostic procedures.