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Invitrogen™ Exosome-Human CD63 Isolation/Detection Reagent (from cell culture media)

Exosome-Human CD63 Isolation/Detection Reagent enables exosome (also called extracellular vesicles and multi-vesicular bodies) detection using techniques such as flow cytometry, electron microscopy, or Western blotting.

Brand:  Invitrogen™ 10606D

329.44 EUR valid until 2024-03-30
Use promo code "21648" to get your promotional price.



Additional Details : Weight : 0.10000kg

Product Code. 14830841

  • 464.00 EUR / 3mL
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Description

Description

Exosome-Human CD63 Isolation/Detection Reagent enables exosome (also called extracellular vesicles and multi-vesicular bodies) detection using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent.

  • Obtain highly pure CD63+ exosomes
  • View sample during handling
  • Easily scalable protocol
  • Detect exosomes via flow cytometry, in less than 1 hour
  • Utilizes Dynabeads magnetic separation technology
  • Allows easy purification of pre-enriched CD63+ exosomes from cell culture media and then move on to detect the purified exosomes via techniques such as flow cytometry, electron microscopy, or Western blotting
  • Bead-bound exosomes can be easily visualized by flow cytometry
  • Monodispersed and relatively large Dynabeads (4.5 μm in diameter) allow for a clear and defined FFC/SSC typically in less than 1 hour
  • Magnetic handling also allows sample to be visualized due to the light brown color of beads
  • Magnets allow for easy separation and purification
  • Sample and bead volume can be easily scaled up or down according to your sample size or downstream applications

Good Mixing is Critical

  • For successful exosome isolation, it is important to use a mixer that tilts and rotates to ensure that beads do not settle in tube
  • Avoid end-over end rotation for small sample volumes (e.g., 100 μL)
  • Please see user manual below for more guidelines
Specifications

Specifications

Cells in culture
Isolation/Detection Reagent
Cell Culture Supernatants
4.5 μm
Store at 2°C to 8°C.
Dynabeads™
Human
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For Research Use Only. Not for use in diagnostic procedures.