Invitrogen™ Exosome-Human CD63 Isolation/Detection Reagent (from cell culture media)

Exosome-Human CD63 Isolation/Detection Reagent enables exosome (also called extracellular vesicles and multi-vesicular bodies) detection using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent.

• Obtain highly pure CD63+ exosomes
• View sample during handling
• Easily scalable protocol
• Detect exosomes via flow cytometry, in less than 1 hour
• Utilizes Dynabeads magnetic separation technology
• Allows easy purification of pre-enriched CD63+ exosomes from cell culture media and then move on to detect the purified exosomes via techniques such as flow cytometry, electron microscopy, or Western blotting
• Bead-bound exosomes can be easily visualized by flow cytometry
• Monodispersed and relatively large Dynabeads (4.5 μm in diameter) allow for a clear and defined FFC/SSC typically in less than 1 hour
• Magnetic handling also allows sample to be visualized due to the light brown color of beads
• Magnets allow for easy separation and purification
• Sample and bead volume can be easily scaled up or down according to your sample size or downstream applications

Good Mixing is Critical

• For successful exosome isolation, it is important to use a mixer that tilts and rotates to ensure that beads do not settle in tube
• Avoid end-over end rotation for small sample volumes (e.g., 100 μL)
• Please see user manual below for more guidelines