missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ AccuPrime™ Taq DNA Polymerase System

The AccuPrime™ Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.

408.00 EUR - 1910.00 EUR

Specifications

Detection Method Primer-probe
GC-Rich PCR Performance Low
PCR Method 1-step RT-qPCR, Multiplex qPCR
Polymerase AccuPrime Taq DNA Polymerase
Reaction Speed Standard
View More Specs

Includes: AccuPrime™ Taq DNA Polymerase is provided in a 200 reaction kit. Each kit includes: AccuPrime™ Taq DNA Polymerase (100μL, at 2U/μL); 10X AccuPrime™ PCR Buffer I (500μL); 10X AccuPrime™ PCR Buffer II (500μL); 50mM Magnesium Chloride (500μL); 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200bp–4 kb). 10X AccuPrime™ PCR Buffer contains 200mM Tris-HCl (pH 8.4), 500mM KCl, 15mM MgCl2, 2mM dGTP, 2mM dATP, 2mM dTTP, 2mM dCTP, thermostable AccuPrime™ protein, 10% glycerol.

Products 2
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
10257142
View Documents Promotion Details
Invitrogen™
12339016
200 Reactions
408.00 EUR
Each
View alternative products
Save Up to 
»
In Stock
to see stock.
Please to purchase this item. Need a web account? Register with us today!
10083062
View Documents Promotion Details
Invitrogen™
12339024
1000 Reactions
1910.00 EUR
Each
View alternative products
Save Up to 
»
In Stock
to see stock.
Please to purchase this item. Need a web account? Register with us today!
Description

Description

The AccuPrime™ Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases (see figure). Platinum™ anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime™ Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C.

Advantages of AccuPrime™ Taq DNA Polymerase:
Specificity—control mispriming at every cycle to get single-band PCR products
Ease of use—minimize reaction optimization and primer set redesign
Convenience—conveniently assemble reactions at room temperature

AccuPrime™ buffers
The 10X AccuPrime™ buffers contain thermostable AccuPrime™ protein, Mg++, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime™ PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).

Applications
AccuPrime™ Taq DNA Polymerase can be used for a wide variety of applications, including multiplex PCR, TOPO TA Cloning™, and allele-specific amplifications.

Order Info

Shipping Condition: Approved for shipment on wet or dry ice

Specifications

Specifications

Primer-probe
1-step RT-qPCR, Multiplex qPCR
Standard
Wet or Dry Ice
10X
Built-In Hot Start
Separate Components
Low
AccuPrime Taq DNA Polymerase
DNA Polymerase System
Hot-start PCR
2X
3'-A
5 kb or less
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.