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Invitrogen™ AccuPrime™ Taq DNA Polymerase System
The AccuPrime™ Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.
408.00 EUR - 1910.00 EUR
Specifications
Detection Method | Primer-probe |
---|---|
GC-Rich PCR Performance | Low |
PCR Method | 1-step RT-qPCR, Multiplex qPCR |
Polymerase | AccuPrime Taq DNA Polymerase |
Reaction Speed | Standard |
Includes: AccuPrime™ Taq DNA Polymerase is provided in a 200 reaction kit. Each kit includes: AccuPrime™ Taq DNA Polymerase (100μL, at 2U/μL); 10X AccuPrime™ PCR Buffer I (500μL); 10X AccuPrime™ PCR Buffer II (500μL); 50mM Magnesium Chloride (500μL); 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200bp–4 kb). 10X AccuPrime™ PCR Buffer contains 200mM Tris-HCl (pH 8.4), 500mM KCl, 15mM MgCl2, 2mM dGTP, 2mM dATP, 2mM dTTP, 2mM dCTP, thermostable AccuPrime™ protein, 10% glycerol.
Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
10257142
|
Invitrogen™
12339016 |
200 Reactions |
408.00 EUR
Each |
In Stock Log in to see stock. |
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10083062
|
Invitrogen™
12339024 |
1000 Reactions |
1910.00 EUR
Each |
In Stock Log in to see stock. |
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Description
The AccuPrime™ Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases (see figure). Platinum™ anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime™ Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C.
Advantages of AccuPrime™ Taq DNA Polymerase:
• Specificity—control mispriming at every cycle to get single-band PCR products
• Ease of use—minimize reaction optimization and primer set redesign
• Convenience—conveniently assemble reactions at room temperature
AccuPrime™ buffers
The 10X AccuPrime™ buffers contain thermostable AccuPrime™ protein, Mg++, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime™ PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).
Applications
AccuPrime™ Taq DNA Polymerase can be used for a wide variety of applications, including multiplex PCR, TOPO TA Cloning™, and allele-specific amplifications.
Order Info
Shipping Condition: Approved for shipment on wet or dry ice
Specifications
Primer-probe | |
1-step RT-qPCR, Multiplex qPCR | |
Standard | |
Wet or Dry Ice | |
10X | |
Built-In Hot Start | |
Separate Components |
Low | |
AccuPrime Taq DNA Polymerase | |
DNA Polymerase System | |
Hot-start PCR | |
2X | |
3'-A | |
5 kb or less |
For Research Use Only. Not for use in diagnostic procedures.