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Thermo Scientific™ IL-2R ELISA Kit, Human

Product Code. 15388194
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Quantity:
192 Assays
Unit Size:
Pack of 2
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Product Code. 15388194

Brand: Thermo Scientific™ EH2IL2R2

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Description

IL-2R ELISA Kit, Human

The Thermo Scientific Human IL-2R ELISA Kit is used to measure s-IL-2R (human soluble interleukin-2 receptor) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatants. Each kit contains two anti-Human sIL-2R capture antibody precoated 96-well Strip Plates and sufficient detection antibody, conjugate, buffers, diluent, TMB and stop buffers, as well as lyophilized sIL-2R standard to analyze several samples.

Features of the Thermo Scientific Human IL-2R ELISA Kit:

  • Target: natural and recombinant human soluble interleukin-2 receptor (sIL-2R); also called CD25
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 0 to 6500 U/mL†
  • Sensitivity: < 24 U/mL†
  • Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 2.5 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, GM-CSF, IFNγ, IFNα, TNFβ, or mouse IL-2.

    † Note: Standards in this ELISA are calibrated to an internal sIL-2R reference preparation of supernatant from a transfected cell line that secretes recombinant soluble IL-2R. We define 1000 units as the amount of sIL-2R present in 1 mL of an internal reference preparation. Units/mL can be converted to sIL-2R pg/mL using the following formula: (sIL-2R pg/mL) = (sIL-2R U/mL) x (1/0.113).

    This human IL-2 receptor ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of soluble interleukin-2 receptor (sIL-2R, also called CD25) in samples at 50μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kit contains pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

    Includes:
    Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
    • TRUSTED_SUSTAINABILITY

    Specifications

    Assay Range 0 to 6500U/mL
    Assay Sensitivity <24U/mL
    Description IL-2R ELISA Kit, 192 Assays, Human Species, 0 to 6500 U/mLA€  Assay Range, Cytokines and Receptors Protein Family, Colorimetric Detection Method, For Protein Assays and Analysis
    Detection Method Colorimetric
    Immunoassay Kit Format Strip Plate
    Product Type IL-2R ELISA Kit
    Protein Family Cytokines and receptors
    Sample Type Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
    Specificity No significant cross-reaction with human IL-1α±, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, GM-CSF, IFNγ, IFNα±, TNFβ, or mouse IL-2
    Target Species Human
    For Use With (Application) Protein assays and analysis
    Kit Contents Detector antibody, Diluents, HRP Conjugate, Pre-coated plate(s), Standard or calibrator, Stop solution, Substrate, Wash buffer
    Measurement Absorbance at 450nm minus absorbance at 550nm
    Precision Intra-assay CV ≤10%; inter-assay CV ≤10%
    Quantity 192 Assays
    Regulatory Status For research use only (RUO)
    Research Discipline Immunology
    Sample Volume 50 μL
    Storage Requirements 2°C to 8°C
    Target CD25
    Total Assay Time 2.5 hr.
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    For Research Use Only. Not for use in diagnostic procedures.

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