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Invitrogen™ Human IL-2 Matched Antibody Pair

Product Code. 10349254
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10 x 96 Tests
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10349254 10 x 96 Tests Each
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Product Code. 10349254 Supplier Invitrogen™ Supplier No. CHC1243
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    632.00 EUR
    486.64 EUR / 960 reactions
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Description

ELISA

The Human IL-2 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-2. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P60568
Assay Range 31.2-1000 pg/mL
Assay Sensitivity 31.2 pg/mL
Conjugate HRP
Gene Accession No. P60568
Gene Alias IL-2, TCGF, lymphokine, aldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGFaldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF; IL-2; TCGF; lymphokine
Gene Symbols IL2
Immunoassay Kit Format Matched Antibody Pair
Product Type ELISA, ELISA Kit
Quantity 10 x 96 Tests
Sample Type Plasma, Serum, Supernatant
Regulatory Status RUO
Gene ID (Entrez) 3558
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader, Colorimetric Microplate Reader
Includes Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Content And Storage Antibody pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.
Store at 2-8°C.
Gene Symbol IL2
Kit Contents Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Label or Dye Biotin
Protein Aliases IL-2, T-cell growth factor, TCGF
Shipping Condition Wet Ice
Storage Requirements 2-8°C
Target IL-2
Test Time 1 hr 30 min
Total Assay Time 24 hr
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