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Invitrogen™ IFN gamma Monoclonal Antibody (R4-6A2), eBioscience™
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Product Code. 15268057
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Quantity:
500 μg
Unit Size:
500µg
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15268057 500 μg 500µg
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Product Code. 15268057 Supplier Invitrogen™ Supplier No. 14731285

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Description

Rat Monoclonal Antibody

Description: The R4-6A2 antibody reacts with mouse interferon-gamma (IFN-gamma ), a homodimeric 15-20 kDa cytokine secreted by Th1 cells. The R4-6A2 antibody is a neutralizing antibody. IFN-gamma is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity. Applications Reported: The R4-6A2 antibody has been reported for use in cytokine neutralization, and immunoblotting (WB). The purified R4-6A2 antibody can be used for blocking of fluorochrome conjugated R4-6A2 in intracellular flow cytometry experiments to serve as a specificity control. It has also been reported to neutralize IFN gamma binding and biological activity. (Please use Functional Grade purified R4-6A2, Product # 16-7312, in functional assays). Applications Tested: The Functional Grade purified R4-6A2 antibody has been tested for neutralization of mouse IFN gamma bioactivity. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
TRUSTED_SUSTAINABILITY

Specifications

Antigen IFN gamma
Applications Functional Assay, Neutralization
Classification Monoclonal
Clone R4-6A2
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene IFNG
Gene Accession No. P01580
Gene Alias BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g
Gene Symbols IFNG
Host Species Rat
Purification Method Affinity chromatography
Quantity 500 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 15978
Target Species Mouse
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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