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AcroBiosystems GMP Recombinant Human Fibronectin fragment
Description
Fibronectin (Fn) is a glycoprotein whose size ranges from 230 to 270 kDa and usually exists as a dimer, covalently linked by a pair of disulfide bonds at the C-termini. Each monomer consists of three repeating units: 12 Type I, 2 Type II, and 15-17 Type III domains which combined account for 90% of the FN sequence. The extracellular matrix (ECM) plays a key role as both structural scaffold and regulator of cell signal transduction in tissues. Fibronectin is one of the major ECM proteins in the trabecular meshwork (TM). It is found in the sheath material surrounding the elastin tendons that enter the TM from the ciliary muscle within the ciliary body. In times of ECM assembly and turnover, cells upregulate assembly of the ECM protein, FN. FN is assembled by cells into viscoelastic fibrils that can bind upward of 40 distinct growth factors and cytokines. These fibrils play a key role in assembling a provisional ECM during embryonic development and wound healing. Fibril assembly is also often upregulated during disease states, including cancer and fibrotic diseases.
Specifications
Specifications
| Accession Number | Uniprot |
| Characterization | Fibronectin Structure This protein carries no tag. The protein has a calculated MW of 62.6 kDa. The protein migrates as 60 kDa±3 kDa when calibrated against under reducing (R) condition (SDS-PAGE). |
| Format | Liquid |
| Formulation | Supplied as 0.2 μm filtered solution in 12.5 mM Citric acid, pH 6.2 with protectants. Contact us for customized product form or formulation. |
| Quality Control Testing | <0.5 ng/μg of protein tested by ELISA.∣<0.02 ng/μg of protein tested by qPCR.∣Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.∣Sterility testing was performed using the membrane filtration method in compliance with USP &CM and Ph. Eur. 2.6.1.∣Negative |
| Quantity | 2.5 mg |
| Source | GMP Recombinant Human Fibronectin fragment (GMP-FINH18) is expressed from E. coli cells. It contains AA Pro 1361 - Ser 1637 & Ala 1812 - Thr 2107 (Accession # ). Predicted N-terminus: Met Request for sequence |
| Biological Activity | Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) immobilized on SA Chip can bind GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) with a typical affinity constant of 0.799 μM as determined in a SPR assay (Biacore 8K) (QC tested).∣Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82Wa) immobilized on SA Chip can bind GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) with a typical affinity constant of 35.7 nM as determined in a SPR assay (Biacore 8K) (QC tested).∣GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) immobilized on CM5 Chip can bind Heparin Sulfate with a typical affinity constant of 63.7 nM as determined in a SPR assay (Biacore 8K) (QC tested).∣GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) could promote mesenchymal stem cell attachment and proliferation in several passages, comparable with Competitor T. The bioactivity of GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) is similar to Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116).∣GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) could maintain the stemness of MSC in several passages, with high expression of MSC markers CD73, CD90 and CD105 and negative expression of HLA-DR, CD34 and CD45. The stemness ability of GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) is comparable with Competitor T and similar to Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116).∣CD34+ CD45+ hematopoietic cells were seeded on GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) combined with GMP Human DLL4 Protein, Fc Tag (Flagship) (Cat. No. GMP-DL4H27) and GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) coated plates and differentiated for 21 days, then flow cytometry was used to detect the expression of T-cell progenitor markers, CD5 and CD7. GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) combined with GMP Human DLL4 Protein, Fc Tag (Flagship) (Cat. No. GMP-DL4H27) and GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) can induce the formation of CD7+ and CD5+ CD7+ T-cell progenitors. The bioactivity of GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) is similar to Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116), as well as the competitor T. Negative control: w/o Human Fibronectin.∣The SPR based assay shows that GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) is stable at 37°C for 23 days.∣The SPR based assay shows that GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) is stable at 4°C for 180 days.∣The SPR based assay shows that GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) is stable after freezing and thawing 3 times. |
| Species | Human |
| Sterility | Sterile |
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