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AcroBiosystems GMP Human IL-3 Protein

Product Code. 31453794
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31453794 1 mg
31451971 50 μg
31452597 10 mg
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Product Code. 31453794 Supplier AcroBiosystems Supplier No. GMPL03H181mg

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Description

Recombinant Protein

Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The human IL-3 reported to be a monomer, as it is known, contains 133 amino acids residues which is a single non-glycosylated polypeptide. Specifically, human and murine IL-3 share low homology and it does not show activity on murine cells.

Specifications

Accession Number Uniprot
Characterization IL-3 Structure This protein carries no tag. The protein has a calculated MW of 15.2 kDa. The protein migrates as 14 kDa±3 kDa when calibrated against under reducing (R) condition (SDS-PAGE).
Format Powder-GMP
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with protectants. Contact us for customized product form or formulation.
Quality Control Testing <0.5 ng/μg of protein tested by ELISA.∣<0.02 ng/μg of protein tested by qPCR.∣Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.∣Sterility testing was performed using the membrane filtration method in compliance with USP &CM and Ph. Eur. 2.6.1.∣Negative
Quantity 1 mg
Source GMP Human IL-3 Protein (GMP-L03H18) is expressed from E. coli cells. It contains AA Ala 20 - Phe 152 (Accession # ). Request for sequence
Biological Activity GMP Human IL-3 Protein (Cat. No. GMP-L03H18) stimulates proliferation of TF-1 cells. The specific activity of GMP Human IL-3 Protein is >1.00x10^6 IU/mg, which is calibrated against human IL-3 WHO International Standard (NIBSC code: 91/510) (QC tested).∣The activity of GMP Human IL-3 (Cat. No. GMP-L03H18) was higher than other competing products.∣GMP Human SCF Protein (Cat. No. GMP-SCFH25), Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28), GMP Human IL-3 Protein (Cat. No. GMP-L03H18) and GMP Human IL-6 Protein (Cat. No. GMP-L06H27) could support the rapid cell expansion and good cell viability of CD34+ hematopoietic stem cells.∣The Cell based assay shows that GMP Human IL-3 Protein (Cat. No. GMP-L03H18) is stable at 37°C for 24 hours.∣The Cell based assay shows that GMP Human IL-3 Protein (Cat. No. GMP-L03H18) is stable at 4°C for 180 days.∣The Cell based assay shows that GMP Human IL-3 Protein (Cat. No. GMP-L03H18) is stable after freezing and thawing 3 times.∣The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG IL-3.∣MALDI-TOF analysis of GMP Human IL-3 Protein (Cat. No. GMP-L03H18). The labeled peaks at 15209.9 Da and 15080.7 Da correspond to the calculated molecular mass with the N-terminal Met (15213 Da), and without the N-terminal Met (15081 Da), respectively. The minor peak following the major peak is a matrix-associated artifact of the MALDI-TOF (Routinely tested).
Species Human
Sterility Sterile
Content And Storage -20°C to -70°C for 5 years in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. Upon receipt, store it immediately at -20°C or lower for long term storage.
Endotoxin Level Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.
Shipping Condition This product is supplied and shipped with blue ice, please inquire the shipping cost.
Specific Activity The activity of GMP Human IL-3 (Cat. No. GMP-L03H18) was higher than other competing products.
Purity or Quality Grade 0.95
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