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AcroBiosystems GMP Human IL-18 Protein

Product Code. 31452576
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31452576 10 mg
31453187 50 μg
31453194 1 mg
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Product Code. 31452576 Supplier AcroBiosystems Supplier No. GMPL18H1610mg

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Description

Recombinant Protein

Interleukin-18 (IL-18) is a potent proinflammatory cytokine that induces interferon-gamma (IFN-gamma) production from Th1 cells, NK cells and activated macrophages, particularly in the presence of IL-12. IL-18 also functions in developmental regulation of T-lymphocyte helper type I cells and in Fas-mediated cytotoxicity. Suppression of IL-18 activity is being investigated for treatment of chronic inflammatory diseases such as Crohn's disease and rheumatoid arthritis. It acts by inducing heterodimerization of the two subunits of its receptor, IL-18RAlpha and IL-18RBeta shows structural similarity to IL-1.

Specifications

Accession Number Uniprot
Characterization IL-18 Structure This protein carries no tag. The protein has a calculated MW of 18.2 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against under reducing (R) condition (SDS-PAGE).
Format Powder-GMP
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with protectants. Contact us for customized product form or formulation.
Quality Control Testing <0.5 ng/μg of protein tested by ELISA.∣<0.02 ng/μg of protein tested by qPCR.∣Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.∣Sterility testing was performed using the membrane filtration method in compliance with USP &CM and Ph. Eur. 2.6.1.∣Negative
Quantity 10 mg
Source GMP Human IL-18 Protein (GMP-L18H16) is expressed from E. coli cells. It contains AA Tyr 37 - Asp 193 (Accession # ). Request for sequence
Biological Activity GMP Human IL-18 Protein (Cat. No. GMP-L18H16) stimulates secretion of IFN-γ by KG-1 cells. The specific activity of GMP Human IL-18 Protein is >3.00x10^6 IU/mg, which is calibrated against WHO Reference Reagent Interleukin-18 (Human rDNA derived) (NIBSC code: 03/200) (QC tested).∣The activity of GMP Human IL-18 Protein (Cat. No. GMP-L18H16) was higher than other competing products.∣Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14), GMP Human IL-18 Protein (ACROBiosystems, Cat. No. GMP-L18H16) and GMP Human 4-1BB Ligand Protein (ACROBiosystems, Cat. No. GMP-41LH26), in CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3103) for two weeks. The result shows that GMP Human IL-18 Protein can promote the expansion of these cells with a reasonable cell viability. Notably, the cells exhibit better expansion in GMP Human IL-18 Protein (ACROBiosystems).∣Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14), GMP Human IL-18 Protein (ACROBiosystems, Cat. No. GMP-L18H16) and GMP Human 4-1BB Ligand Protein (ACROBiosystems, Cat. No. GMP-41LH26), in CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3103) for two weeks. The result shows that GMP Human IL-18 Protein (ACROBiosystems) can be comparable with the Competitor M IL-18 protein.∣The Cell based assay shows that GMP Human IL-18 Protein (Cat. No. GMP-L18H16) is stable after freezing and thawing 3 times.∣The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG IL-18.∣MALDI-TOF analysis of GMP Human IL-18 Protein (Cat. No. GMP-L18H16), showing a major peak at 18217.2 Da. The minor peak following the major peak is a matrix-associated artifact of the MALDI-TOF (Routinely tested).
Species Human
Sterility Sterile
Content And Storage -20°C to -70°C for 5 years in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. Upon receipt, store it immediately at -20°C or lower for long term storage.
Endotoxin Level Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.
Shipping Condition This product is supplied and shipped with blue ice, please inquire the shipping cost.
Specific Activity The activity of GMP Human IL-18 Protein (Cat. No. GMP-L18H16) was higher than other competing products.
Purity or Quality Grade 0.95
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