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AcroBiosystems GMP Human IL-12 Protein

Product Code. 31451662
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31451662 1 mg
31453488 50 μg
31453189 10 mg
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Product Code. 31451662 Supplier AcroBiosystems Supplier No. GMPL12H231mg

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Description

Recombinant Protein

IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL‐12, produced by macrophages in response to infectious agents, is a central mediator of the cell‐mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

Specifications

Accession Number Uniprot,Uniprot
Characterization This protein carries no tag. The protein has a calculated MW of 58.9 kDa. The protein migrates as 76 kDa±3 kDa when calibrated against under reducing (R) condition (SDS-PAGE) due to glycosylation.
Format Powder-GMP
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with protectants. Contact us for customized product form or formulation.
Quality Control Testing <0.5 ng/μg of protein tested by ELISA.∣<0.02 ng/μg of protein tested by qPCR.∣Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.∣Sterility testing was performed using the membrane filtration method in compliance with USP &CM and Ph. Eur. 2.6.1.∣Negative
Quantity 1 mg
Source GMP Human IL-12 Protein (GMP-L12H23) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 & Arg 23 - Ser 219 (Accession # & ). Predicted N-terminus: Ile 23
Biological Activity GMP Human IL-12 Protein (Cat. No. GMP-L12H23) stimulates secretion of IFN-γ by NK-92. The specific activity of GMP Human IL-12 Protein is >1.00x10^7 IU/mg, which is calibrated against human IL-12 WHO International Standard (NIBSC code: 95/544) (QC tested).∣The activity of GMP Human IL-12 Protein (Cat. No. GMP-L12H23) was higher than other competing products.∣Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14), GMP Human IL-12 Protein (ACROBiosystems, Cat. No. GMP-L12H23) and GMP Human 4-1BB Ligand Protein (ACROBiosystems, Cat. No. GMP-41LH26), in CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3103) for two weeks. The result shows that GMP Human IL-12 Protein (ACROBiosystems) can promote the expansion of these cells with a reasonable cell viability, and can be comparable with the Competitor M IL-12 protein.∣Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14), GMP Human IL-12 Protein (ACROBiosystems, Cat. No. GMP-L12H23) and GMP Human 4-1BB Ligand Protein (ACROBiosystems, Cat. No. GMP-41LH26), in CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3103) for two weeks. The result shows that GMP Human IL-12 Protein (ACROBiosystems) can be comparable with the Competitor M IL-12 protein.∣The Cell based assay shows that GMP Human IL-12 Protein (Cat. No. GMP-L12H23) is stable at 37°C for 24 hours.∣The Cell based assay shows that GMP Human IL-12 Protein (Cat. No. GMP-L12H23) is stable at 4°C for 180 days.∣The Cell based assay shows that GMP Human IL-12 Protein (Cat. No. GMP-L12H23) is stable after freezing and thawing 3 times.∣The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG IL-12.
Species Human
Sterility Sterile
Content And Storage -20°C to -70°C for 5 years in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. Upon receipt, store it immediately at -20°C or lower for long term storage.
Endotoxin Level Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.
Shipping Condition This product is supplied and shipped with blue ice, please inquire the shipping cost.
Specific Activity The activity of GMP Human IL-12 Protein (Cat. No. GMP-L12H23) was higher than other competing products.
Purity or Quality Grade 0.95
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