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Gibco™ Sf-900™ II SFM
Description
Sf-900 II SFM is a serum-free, protein-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells. This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines.
- Superior long-term, high-density growth
- Optimized for recombinant protein production
- Serum-free, protein-free, ready-to-use formulation
- Scalable in bioreactors
Bioproduction, Cell Culture, Insect Cell Culture, Sf9 and Sf21 Cell Culture, Viral Vaccine Production
Informations sur la commande
Shipping Condition: Room temperature
Spécification
Spécification
| Cell Line | Sf21, Sf9 |
| Cell Type | Insect Cell |
| Classification | Protein-free, Serum-free |
| Form | Liquid |
| Product Type | Insect Cell Serum Free Medium (SFM) |
| Serum Level | Serum-free |
| With Additives | Glutamine |
| Product Line | Gibco, Sf-900, SynerGy |
| Quantity | 20 L |
| Shipping Condition | Room Temperature |
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Frequently Asked Questions (FAQs)
It should be okay to thaw the cells into Sf-900 II SFM. This is a richer media compared to the Sf-900 III SFM so the cells would have an easy time adapting. We would recommend taking the cells through 3 passages in the new medium before using them for any experiments as that they have enough time to adapt.
Many antibiotics are suitable for use with insect cells. The following antibiotics are commonly used:
- Penicillin/Streptomycine: 50-100 U/mL; 50-100 µg/mL
- Amphotericin B (Fungizone antimycotic): 0.25 µg/mL
- Gentamicin: 0.5 mL of 10 mg/mL solution in 500 mL media (final concentration: 10 µg/mL)
Heat inactivation is not necessary. Our team has routinely used serum that has not been heat-inactivated, and we have not observed any effect on cell growth or morphology.
Many cells do not require heat-inactivated FBS. Some cells prefer heat-inactivated FBS. For instance, we use heat-inactivated FBS for our insect cell lines, i.e., Sf9 and Sf21 cells.
Yes, we offer a variety of serum-free insect media. Please go here (http://www.thermofisher.com/us/en/home/life-science/cell-culture/insect-cell-culture/insect-cell-culture-misc/serum-free-media.html?icid=cvc-insect-media-c2t2) to view the media we offer and the differences between them.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
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