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Gibco™ Ham's F-12 Nutrient Mix
Descripción
Requires
Supplementation
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Ham's F-12 is modified as follows:
With: L-glutamine, Phenol Red
The complete formulation is available.
Using F-12
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Información de órdenes
Shipping Condition: Room Temperature
Especificaciones
Especificaciones
| Cell Line | CHO, COS-7, and rat prostate epithelial cells |
| Cell Type | Primary Rat Astrocytes |
| Classification | Animal Origin-free |
| Concentration | 1 X |
| Form | Liquid |
| Product Type | Ham's F-12 Nutrient Mixture |
| Sterility | Sterile-filtered |
| Sterilization Method | Sterile-filtered |
| With Additives | Glutamine, Phenol Red, Sodium Pyruvate |
| Without Additives | No HEPES |
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