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Gibco™ Grace's Insect Medium, unsupplemented, powder
Useful during transfection of insect cells or for protein labeling studies with methionine
Brand: Gibco™ 11300027
Additional Details : Weight : 10.00000kg
Requires : Requires supplementation with 10% Fetal Bovine Serum (FBS), 3.33g/L lactalbumin hydrolysate and 3.33g/L yeastolate before use as a growth medium as well as ambient CO2 in order to maintain physiological pH. Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation.
Description
Widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). Was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. It is modified as follows: with L-glutamine, without Lactalbumin hydrolysate, Yeastolate, Sodium bicarbonate. It does not contain lactalbumin hydrolysate and yeastolate commonly found in the supplemented version
Cell Culture, Insect Cell Culture, Sf9 and Sf21 Cell Culture
Order Info
Shipping Condition: Room temperature
Compliance
Manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Specifications
| Storage conditions: 2-8°C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
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| Powder | |
| Glutamine | |
| Gibco™ | |
| Room Temperature |
| Insect Cell | |
| Grace's Insect Medium | |
| No HEPES, No Phenol Red, No Sodium Pyruvate, No Lactalbumin Hydrolysate, No Yeastolate, No Sodium Bicarbonate | |
| 10 x 1 L |
For Research Use Only. Not for use in diagnostic procedures.