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  6. Gibco™ Geltrex™ Flex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix

Gibco™ Geltrex™ Flex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix

Product Code. 30565268
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Product Code. 30565268 Supplier Gibco™ Supplier No. A4000046801

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46.08 EUR valid until 2026-06-30
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Description

Geltrex Flex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix is a soluble basement membrane matrix purified from EHS tumors. Each lot is tested with iPSCs and hESC cells to ensure reliability and consistency in hESC/iPSC studies.

Gibco Geltrex Flex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane that is purified from Engelbreth-Holm-Swarm (EHS) tumor cells. The major components of Geltrex Flex matrix include laminin, collagen IV, entactin, and heparan sulfate proteoglycan.

Routinely used for attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), every lot of hESC-qualified Geltrex Flex matrix is quality control tested using hESCs and iPSCs to help ensure support of stem cell culture. For a neural induction of hESC and iPSC protocol, see Induction of Neural Stem Cells from Human Pluripotent User Guide (Pub. No. MAN0008031).

Features of Geltrex Flex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix

  • Each lot tested with iPSCs and H9 hESCs for at least five passages to help ensure reliability and consistency in hESC/iPSC studies
  • Each lot tested for the absence of lactose dehydrogenase elevating virus (LDEV), mycoplasma, and a broad panel of viruses and microorganisms
  • Lots tested for endotoxin, maintenance of gel stability, and HUVEC tube formation
  • Growth factor–reduced to improve lot-to-lot consistency
  • Formulated without phenol red to minimize potential for estrogen-like effects
  • Provided in recyclable glass vials
  • 1-mL, 5-mL, and 10-mL sizes for maximum flexibility. Use only what you need. Reduce need for extensive aliquoting and unwanted freeze-thaws.

For the culture of primary cells, such as epithelial cells, endothelial cells, and smooth muscle cells, and angiogenesis assays, neurite outgrowth assays, or bile canaliculi formation assays with polarized hepatocytes, standard Geltrex Flex matrix can be used.

For the culture of tumoroids and other organoids, Geltrex Flex LDEV-Free Organoid-Qualified Reduced Growth Factor Basement Membrane Matrix is recommended. Each lot of organoid-qualified Geltrex Flex matrix is tested and supports the culture of mouse intestinal organoids, mouse liver ductal organoids, and mouse airway organoids, as well as expansion of mouse small intestinal organoids for more than five passages with typical organoid budding morphology.

TRUSTED_SUSTAINABILITY

Specifications

Cell Type Human induced pluripotent stem cells (iPSC), human embryonic stem cells (hESC), HUVEC, primary epithelial cells, neurons, endothelial cells, smooth muscle cells, hepatocytes.
Classification Reduced Growth Factor
Concentration 9 to 13 mg/mL
Form Frozen
Product Type Basement Membrane Matrix
Serum Level Serum-free
Sterility Sterile
Without Additives No Phenol Red
Quantity 1 mL
Shipping Condition Dry Ice
Endotoxin Level ≤ 4.5 EU/mL
Purity or Quality Grade Cell Culture Grade
Species Mouse
Culture Type Adherent Cell Culture, Mammalian Cell Culture, Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
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Frequently Asked Questions (FAQs)

Do you still recommend using Gibco Vitronectin as the matrix for Gibco Essential 8 Flex Medium?

Both Gibco Vitronectin (VTN-N) Recombinant Human Protein, Truncated (Cat. No. A400457 and A400458) and Gibco Geltrex Flex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (Cat. No. A4000046801, A4000046802, or A4000046803) have been successfully used with Essential 8 Flex Medium.

What substrate should I use for cardiomyocyte differentiation?

We recommend using Gibco Geltrex Flex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (Cat. No. A4000046801, A4000046802, or A4000046803) or Gibco Vitronectin (VTN-N) Recombinant Human Protein, Truncated (Cat. No. A400457 or A400458) for xeno-free applications.

You recommend Geltrex matrix as the matrix for routine culture of StemFlex Medium. Can I also use Vitronectin or rhLaminin-521?

Yes. We recommend following the coating instructions for Vitronectin or rhLaminin-521 and using the culture and passaging recommendations in the StemFlex protocol.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for human fibroblasts?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of both neonatal and adult human dermal fibroblasts. For fibroblasts, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 16-20). On Day 7, you may use rhVTN-N (Cat. No. A400457 or A400458), Geltrex Flex matrix (Cat. No. A4000046801, A4000046802, or A4000046803), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, we recommend that you feed reprogrammed fibroblasts every-other-day with StemFlex Medium.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for blood cells?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of CD34+ blood cells. For CD34+ cells, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 39-44). On Day 3, you can utilize rhVTN-N (Cat. No. A400457 or A400458), Geltrex Flex matrix (Cat. No. A4000046801, A4000046802, or A4000046803), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, reprogrammed CD34+ cells should be fed every-other-day with StemFlex Medium.

For Research Use Only. Not for use in diagnostic procedures.

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