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Thermo Scientific™ Mph1103I (NsiI) (10 U/μL)
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Description
Lambda DNA digested with Mph1103I (NsiI), 0.7% agarose, 14 cleavage sites
conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
5'..A▵T G C A▵T...3'
3'..T▵A C G T▵A...5'
Conditions for 100% Activity:
- 1X Buffer R: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
- Incubate at 37°C
Storage Buffer:
- Mph1103I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
Methylation Effects:
- Dam: never overlaps —no effect
- Dcm: never overlaps —no effect
- CpG: never overlaps —no effect
- EcoKI: never overlaps —no effect
- EcoBI: may overlap —effect not determined
Compatible Ends:
- Alw21I, PstI, SdaI, SduI
Specifications
Specifications
| Concentration | 10 U/μL |
| Methylation Sensitivity | Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive |
| Enzyme | Mph1103I (NsiI) |
| Compatible Buffer | 10X Buffer R |
| Sensitive to Heat Inactivation | Yes |
| Optimal Reaction Temperature | 37°C |
| Type IIS RE | No |
| Quantity | 1,000 units |
| Product Type | Restriction Enzyme |
| Research Category | Traditional Cloning |
For Research Use Only. Not for use in diagnostic procedures.
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