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Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Código de producto. 11863923 Tienda Thermo Scientific Productos
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No. of Reactions:
1,000 Reactions
200 reactions
4,000 Reactions
Tamaño de la unidad:
1000 reactions
200 reactions
4000 reactions
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Código de producto. No. of Reactions unitSize
11863923 1,000 Reactions 1000 reactions
11853923 200 reactions 200 reactions
11340760 4,000 Reactions 4000 reactions
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Código de producto. 11863923 Proveedor Thermo Scientific™ N.º de proveedor K0252

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Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.

  • Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity - detects low copy number targets
  • Wide linear range - accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience - ready-to-use 2X master mix
TRUSTED_SUSTAINABILITY

Especificaciones

Concentration 2X
Content And Storage Contains:
• 10 x 1.25 mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 1000 x 25 μL reactions.
• 250 μL ROX solution, 50 μM
• 10 x 1.25 mL Nuclease-Free Water

Store at -20°C.
Detection Method SYBR
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase Taq DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Product Line Maxima
Product Type Real Time PCR SYBR Master Mix
Quantity 1,000 reactions
Sufficient For 1000 Reactions at 25 μL
For Use With (Application) Gene Expression
No. of Reactions 1,000 Reactions
Sample Type DNA, dsDNA
Volume 10 x 1.25 mL
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For Research Use Only. Not for use in diagnostic procedures.

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