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  6. Thermo Scientific™ Pfu DNA Polymerase

Thermo Scientific™ Pfu DNA Polymerase

Product Code. 10631231 Shop All Thermo Scientific Products
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Description:
Pfu DNA Polymerase
Pfu DNA Polymerase (recombinant)
Quantity:
100U
500U
Unit Size:
100 units
500 units
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Product Code. 10631231

Brand: Thermo Scientific™ EP0571

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Description

Optimize PCR applications requiring high fidelity with this highly thermostable DNA polymerase.

Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’ direction. Pfu DNA Polymerase also exhibits 3’→5’ exonuclease (proofreading) activity that enables the polymerase to correct nucleotide incorporation errors. It has no 5’→3’ exonuclease activity.

Highlights

  • Eight times more accurate than Taq DNA polymerase
  • Highly thermostable – remains 95% active after 2 hours incubation at 95°C
  • Generates blunt-end PCR products
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
  • Increased PCR yields with native Pfu DNA Polymerase
TRUSTED_SUSTAINABILITY

Specifications

Description Pfu DNA Polymerase
Quantity 100U
For Use With (Application) High-fidelity PCR, Standard PCR, Cloning, RT-PCR, Site-directed Mutagenesis
Thermostability remains 95% active after 2 hours incubation at 95°C
Includes 0.6mL 10X Pfu Buffer with (NH4)2SO2, 0.60mL 10X Pfu Buffer with MgSO4 and 0.06mL 25mM MgSO4
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