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Descripción
Thermo Scientific™ dUTP (2'-deoxyuridine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. The nucleotide has greater than 99% purity and is free of nuclease activities and human and E. coli DNA. It is designed for many different molecular biology applications.
Features
- Greater than 99% purity confirmed by HPLC
- Free of human and E. coli DNA
- Highly stable
Applications
- Long range PCR (40 kb)
- cDNA synthesis and RT-PCR
- Real-time PCR
- Standard PCR
- High fidelity PCR
- Primer extension.
Note: dUTP is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.
Especificaciones
Especificaciones
| Concentration | 100 mM |
| Content And Storage | dUTP Solution, 100 mM (0.25 mL) Store at –20°C. |
| Form | Liquid |
| PCR Method | 2-step RT-qPCR, Standard PCR |
| Polymerase | DNA Polymerase |
| Label or Dye | Unlabeled |
| Purity or Quality Grade | >99% |
| Quantity | 0.25 mL |
| Shipping Condition | Dry Ice |
| For Use With (Application) | Standard PCR, High-fidelity PCR, RT-PCR, Real Time PCR (qPCR) |
For Research Use Only. Not for use in diagnostic procedures.
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