EVOS™ M7000 Imaging System, High-Content Analysis Package

Description

• Combines the fully automated, inverted, multi-channel fluorescence and transmitted light imaging system with Celleste 6.0 Image Analysis Software
• This software offers a full-featured image analysis suite with protocols optimized to deliver the most relevant data for a range of popular live and fixed cell-based assays
• The EVOS M7000 system was developed to meet today’s expectations for publication-quality images and data with just a few clicks using its powerful and time-saving acquisition tools with enhanced autofocus algorithms and automated routines
• For microwell plate assays, time-lapse live cell imaging, area scanning in time lapse mode, and Z-stacks in scan mode
• Celleste 6.0 software allows users to customize image analysis to meet their specific needs or take advantage of preconfigured analysis templates optimally designed to segment and classify images from a range of common cell-based assays
• The union of the Celleste 6.0 software and EVOS microscope empowers users to easily transition from performing traditional microscopy to high-content analyses

The EVOS M7000 High-Content Analysis packages offer:

• Highly configurable microscope—with a five-position objective turret and the ability to simultaneously acquire images in four fluorescence channels plus transmitted light with magnification from 1.25X to 100X, the optical system can be easily configured to meet your sample needs
• Publication-ready imagery in a few clicks—including autofocus, stitching, tiling, Z-stack capability, time-lapse imaging and single-click multichannel capture
• Intuitive acquisition interface—easily program the EVOS M7000 system to run well-plate scanning, time lapse experiments, and tile-stitch/montage area scans in Z-stack and/or time-lapse modes in your vessel of choice
• Streamlined image analysis—Celleste 6.0 icon-based wizard-drive workflow eliminates guesswork when using the preconfigured and optimized analysis templates for common applications to help derive the most relevant results from image sets

System and hardware specifications

• Optics: infinity‐corrected optical system; RMS‐threaded objectives with 45-mm parfocal distance. Imaging mode: fluorescence, brightfield, color brightfield, and phase contrast
• Illumination: five-position chamber for four fluorescence light cubes plus brightfield; light cubes with integrated hard coated filter set and LED light source with >50,000 hour life; broad selection of standard and specialty light cubes
• Imaging methods: single-color, multi-color, area scan with montage or tile-stitch, time lapse, Z-stacking, movie capture
• Objective capacity: five‐position turret
• Objectives (not included): wide selection of high‐quality LWD and coverslip‐corrected objectives
• Condenser: 60-mm long working distance condenser, four-position turret with a clear aperture and three phase annuli
• Stage: motorized X/Y scanning stage; travel range 120 mm x 80 mm with sub‐micron resolution, drop‐in inserts to receive vessel holders and lock-down holders to fix sample in place during long scans
• Focus mechanism: automated focus mechanism with sub‐micron resolution
• LCD display: 23-inch high‐resolution touch screen color monitor (also fully controllable via mouse); 1920 x 1080 pixel resolution
• Cameras: high-sensitivity 3.2 MP (2048 x 1536) monochrome CMOS sensor with 3.45 μm pixel resolution