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  5. Invitrogen™ Human Endothelin-1 (ET-1) ELISA Kit

Invitrogen™ Human Endothelin-1 (ET-1) ELISA Kit

Product Code. 17855117
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10 x 96 Tests
96 Tests
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17855117 10 x 96 Tests Pack of 10
15755293 96 Tests Each
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Product Code. 17855117 Supplier Invitrogen™ Supplier No. EIAET1X10

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Description

ELISA

The Endothelin-1 (ET-1) Multispecies ELISA quantitates Endothelin-1 in human, bovine, procine, dog, rat, and mouse serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Endothelin-1. Principle of the method The Endothelin-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P05305
Assay Range 0.781 to 100 pg/mL
Assay Sensitivity 0.579 pg/mL
Description Endothelin-1 (ET-1) ELISA Kit
Assay Multiplex Assay on the Luminex Platform
Conjugate HRP
Detection Method Fluorescence
Product Type ELISA
Sample Type Plasma, Serum, Supernatant, Urine
Sufficient For 10 x 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 1906
Gene Symbol EDN1
Interassay CV 3.7%
Intraassay CV 3.3
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Extraction Solution
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Research Discipline Cell Signaling
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 2.5 μL, Urine 50 μL
Storage Requirements -20°C
Target Endothelin 1
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 30 mins
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