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STEMCELL Technologies EasySep™ Human Memory B Cell Isolation Kit

Product Code. 15845427
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2 x 109 cells
Unit Size:
1 set
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15845427 2 x 109 cells 1 set
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Product Code. 15845427 Supplier STEMCELL Technologies Supplier No. 17864

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Immunomagnetic positive selection of human memory B cells

  • The EasySep™ Human Memory B Cell Isolation Kit is a two-step method designed to isolate CD27+ B cells from fresh or previously frozen peripheral blood mononuclear cells
  • First, CD27+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™
  • Then, bound magnetic particles are removed from the EasySep™-isolated CD27+ cells, and unwanted non-B cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™
  • The final isolated fraction contains highly purified CD27+ memory B cells that are immediately ready for downstream applications
  • An optional protocol allows for the isolation of naïve B cells in parallel for use in functional studies

Spezifikation

Cell Type B Cells
For Use With (Application) Cell Isolation
Quantity 2 x 109 cells
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