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Thermo Scientific™ DreamTaq PCR Master Mixes (2X)
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Thermo Scientific DreamTaq PCR Master Mix (2X), available in colorless and green formats, is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR setup.
The master mix retains all features of DreamTaq DNA Polymerase and is capable of robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required.
DreamTaq Green PCR Master Mix (2X) additionally includes 2X DreamTaq Green Buffer with a density reagent and two dyes for monitoring electrophoresis progress.
Features
- Robust amplification with minimal optimization
- High yields of PCR products
- Higher sensitivity compared to conventional Taq DNA polymerase
- Amplification of targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
- Incorporates modified nucleotides, but does not incorporate dUTP
- Direct loading of PCR product on gel with green format
Applications
- Routine PCR amplification of DNA fragments up to 6 kb
- High throughput PCR
- Genotyping
Tekniske data
Tekniske data
| Concentration | 2X |
| Content And Storage | • 2X DreamTaq Green PCR Master Mix, 4 x 1.25 mL • Nuclease-free water, 4 x 1.25 mL Store at -20°C. |
| GC-Rich PCR Performance | Low |
| Polymerase | DreamTaq DNA Polymerase |
| Reaction Speed | Standard |
| Quantity | 4 x 1.25 mL |
| Shipping Condition | Dry Ice |
| For Use With (Application) | Standard PCR |
| Fidelity (vs. Taq) | 1X |
| Hot Start | No |
| Vis mere |
For Research Use Only. Not for use in diagnostic procedures.
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