missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. 15392076

IBA Lifesciences CD45RA Fab Streptamer™ Isolation Kit MB

Product Code. 15392076 Shop All IBA Lifesciences Products
Change view
Click to view available options
Quantity:
1 Kit
Unit Size:
1 set
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
15392076 1 Kit 1 set
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 15392076 Supplier IBA Lifesciences Supplier No. 68000208

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

CD45RA Fab Streptamer Isolation Kit MB is for reversible cell isolation delivering label-free target cells.

  • Strep-Tactin™ Magnetic Microbeads for Fab Streptamers™; 2 vials for 5 x 108 cells each
  • CD45RA Fab-Strep, human for isolation of T-cells; 2 vials for 5 x 108 cells
  • Biotin stock solution for Streptamer™ technology; 2 x 1 mL
  • Buffer IS, Streptamer™ Washing Buffer, 10x concentrated; 50 mL
  • Microbeads are intended for use with a permanent magnet for manual cell isolation; we recommend our StrepMan magnet for 2 x 15 mL and 2 x 50 mL Falcon tubes.
  • Principle of Selection: Isolation of human CD45RA T cells is performed by positive selection.
  • PBMCs are labeled with magnetic CD45RA Fab Streptamers™.
  • Labeled cells are isolated in a strong magnet (StrepMan, horizontal position) where they migrate toward the tube wall on the side of the magnet.
  • This CD45RA positive cell fraction is collected and cells are liberated from all labeling reagents by addition of biotin in a strong magnet (StrepMan, upright position).
  • The magnetic Streptamers™ migrate toward the tube wall and the label-free cells remain in the supernatant.
  • Biotin is removed by washing.
  • The resulting cell preparation is highly enriched with CD45RA+ T cells with a purity of more than 90%.
  • No depletion steps and no columns are needed.

Specifications

Product Type Cell Isolation Kit
Donor Source PBMCs
Sufficient For 1 x 109 cells
Quantity 1 Kit
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.