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IBA Lifesciences CD3 Fab Streptamer™ Isolation Kit MB

Product Code. 15342076 Shop All IBA Lifesciences Products
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Product Code. 15342076 Supplier IBA Lifesciences Supplier No. 68000201

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Description

CD3 Fab Streptamer Isolation Kit MB is for reversible cell isolation delivering label-free target cells.

  • Strep-Tactin™ Magnetic Microbeads for Fab Streptamers™; 2 vials for 5 x 108 cells each
  • CD3 Fab-Strep, human for isolation of T-cells; 1 vial for 1 x 109 cells
  • Biotin stock solution for Streptamer™ technology; 2 x 1 mL
  • Buffer IS, Streptamer™ Washing Buffer, 10x concentrated; 50 mL
  • Microbeads are intended for use with a permanent magnet for manual cell isolation; we recommend our StrepMan magnet for 2 x 15 mL and 2 x 50 mL Falcon tubes.
  • Principle of Selection: Isolation of human CD3+ T cells is performed by positive selection.
  • PBMCs are labeled with magnetic CD3 Fab Streptamers™.
  • Labeled cells are isolated in a strong magnet (StrepMan, horizontal position) where they migrate toward the tube wall on the side of the magnet.
  • This CD3 positive cell fraction is collected and cells are liberated from all labeling reagents by addition of biotin in a strong magnet (StrepMan, upright position).
  • The magnetic Streptamers™ migrate toward the tube wall and the label-free cells remain in the supernatant.
  • Biotin is removed by washing.
  • The resulting cell preparation is highly enriched with CD3+ T cells with a purity of more than 90%.
  • No depletion steps and no columns are needed.

Specifications

Description Isolation of label-free cells
Product Type Cell Isolation Kit
Donor Source PBMCs
Sufficient For 1 x 109 cells
Quantity 1 Kit
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