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Invitrogen™ TaqMan™ Fast Cells-to-CT™ Kit
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Artikelnummer. 10712697
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Quantity:
100 reactions
Pakningsstørrelse:
100 reactions
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27969” to get your promotional price
Artikelnummer. 10712697 Leverandør Invitrogen™ Leverandørnr. 4399003

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1680.00 EUR valid until 2026-06-26
BEST PRICE on Promo! Use promo code "27969" to get your promotional price.
Denne vare kan ikke returneres. Se returpolicy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27969” to get your promotional price

Beskrivelse

Indeholder

Stop Solution: 500μL tube; DNase I: 55μL tube; 20X RT Enzyme Mix: 275μL tube; Lysis Solution: 5.5mL bottle; 2X RT Buffer: 5.5mL bottle; TaqMan Fast Universal PCR Master Mix: 4 x 1.25mL bottle

The TaqMan™ Fast Cells-to-CT™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.

  • Complete: Optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, RT buffer, and TaqMan™ Fast Universal PCR Master Mix
  • Fast: Prepare samples at room temperature in 7 minutes, including DNase treatment; TaqMan™ Fast Universal PCR Master Mix delivers real-time PCR results in ∼35 minutes
  • Convenient: Lyse samples in tube or directly in 96- or 384-well culture plates
  • Robust: Perform gene expression analysis on 10 to 100,000 cells per sample, with results equivalent to those from purified RNA
  • Efficient: Contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

Ordreinfo

Shipping Condition: Dry ice

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Tekniske data

Content And Storage

Contains:
• Stop Solution: 500 μL tube
• DNase I: 55 μL tube
• 20X RT Enzyme Mix: 275 μL tube
• Lysis Solution: 5.5 mL bottle
• 2X RT Buffer: 5.5 mL bottle
• TaqMan™ Fast Universal PCR Master Mix: 4 x 1.25 mL bottle

Store Stop Solution, DNase I, and 20X RT Enzyme Mix at -20°C.
Store Lysis Solution, 2X RT Buffer, and TaqMan™ Fast Universal PCR Master Mix at 4°C.
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance Low
PCR Method 2-step RT-qPCR
Polymerase DNA Polymerase
Reaction Speed Fast
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Passive Reference Dye ROX (Pre-mixed)
Product Line Ambion, Cells-to-CT, TaqMan
Product Type Fast Cells-to-C Kit
Quantity 100 reactions
Reverse Transcriptase M-MLV
Shipping Condition Dry Ice
Sufficient For 100 Reactions
For Use With (Application) Gene Expression
No. of Reactions 100 reactions
Sample Type Cell
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For Research Use Only. Not for use in diagnostic procedures.

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