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Abnova ES-E14TG2a (Mouse pluripotent embryonic stem cell) whole cell lysate (nondenatured)

Product Code. 16029615
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Quantity:
50 μg
Unit Size:
50µg
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16029615 50 μg 50µg
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Product Code. 16029615 Supplier Abnova Supplier No. L045V2.50ug

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Description

  • Maintained in the undifferentiated state using specialized culturing protocols
  • Monitored for undifferentiated cell morphology
  • Analyzed using Flow Cytometry against specific cell marker
  • Coommasie Blue Staining of total cell lysate in SDS-PAGE

Western Blotting, Immunoprecipitation

Specifications

For Use With (Application) Immunoprecipitation, Western Blot
Tissue Embryonic
Description Whole cell lysate (non-denatured)
Lysis Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quality Control Testing 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity 50 μg
Storage Buffer In modified RIPA Lysis Buffer.
Host Species Mouse
Concentration 4 mg/mL
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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