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Gibco™ AAV-MAX Helper-Free AAV Production System Kit

Product Code. 17496222
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17496222 Sufficient for 1 L of Culture Each
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Product Code. 17496222 Supplier Gibco™ Supplier No. A51217

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Description

The AAV-MAX Helper-Free AAV Production System Kit is a scalable, high-titer platform for suspension AAV production.

The Gibco AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production. It is based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium. Optimal viral titers are mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel production enhancer. The components are designed to work synergistically, resulting in maximal titers and eliminating the need to optimize reagents and protocols.

Features of the AAV-MAX Helper-Free AAV Production System include:

  • Components work synergistically to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified)
  • Suspension-based system has scalable protocols from shake flask to bioreactor scale
  • Simplified workflow—streamlined protocol using helper virus-free triple transfection
  • Clonal, 293F-derived, high-producer cell line
  • Robust transfection protocols are adaptable to large-scale transfection
  • The components included in the AAV-MAX System Kit are:
    • 1 vial of Viral Production Cells 2.0
    • 1 L of Viral Production Medium
    • 1 AAV-MAX Transfection Kit for 1 L of production
    • 100 mL Viral-Plex Complexation Buffer
    • 100 mL AAV-MAX Lysis Buffer
    • 100 mL GlutaMAX Supplement

All components are also available for purchase separately.

TRUSTED_SUSTAINABILITY

Especificaciones

Cell Line Viral Production Cells 2.0
Cell Type Kidney (Embryonic)
Product Type AAV Production System
Sterility Sterile
For Use With (Application) AAV Production
Product Line AAV-MAX
Quantity Sufficient for 1 L of Culture
Content And Storage • 1 vial Viral Production Cells 2.0
• 1 L Viral Production Medium
• 1 AAV-MAX Transfection Kit for 1 L production
• 100 mL Viral-Plex Complexation Buffer
• 100 mL AAV-MAX Lysis Buffer
• 100 mL GlutaMAX Supplement

Storage conditions vary
Culture Type Suspension Cell Culture

For Research Use Only. Not for use in diagnostic procedures.

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