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A-431 (human epidermoid carcinoma) Nuclear extract lysate, Non-denatured; Abnova

Product Code. 16029545
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Quantity:
50 μg
Unit Size:
50µg
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16029545 50 μg 50µg
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Product Code. 16029545 Supplier Abnova Supplier No. L015V4.50ug

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Description

Non-denatured nuclear extract lysate of A-431 (human epidermoid carcinoma)

  • Tissue: Epidermis
  • Host: Human
  • Lysis Buffer:
    • Buffer A: 10mM HEPES pH 7.9, 1.5mM MgCl2, 10mM KCl, 0.5mM DTT.
    • Buffer C: 20mM HEPES pH 7.9, 25%(v/v) Glycerol , 0.42M NaCl , 1.5mM MgCl2, 0.2mM EDTA, 0.5mM DTT & 0.5mM PMSF
    • Buffer D : 20mM HEPES pH 7.9, 20%(v/v) glycerol, 50mM KCl, 0.2mM EDTA, 0.5mM DTT & 0.5mM PMSF
  • Storage buffer: In buffer D

Applications:

Western Blotting, Immunoprecipitation

Specifications

For Use With (Application) Immunoprecipitation, Western Blot
Tissue Epidermis
Description Nuclear extract cell lysate (non-denatured)
Lysis Buffer Buffer A: 10mM HEPES pH 7.9, 1.5mM MgCl2, 10mM KCl, 0.5 mM DTT. Buffer C: 20mM HEPES pH 7.9, 25%(v/v) Glycerol , 0.42M NaCl , 1.5mM MgCl2, 0.2 mM EDTA, 0.5 mM DTT & 0.5 mM PMSF. Buffer D : 20mM HEPES pH 7.9, 20%(v/v) glycerol, 50mM KCl, 0.2 mM EDTA, 0.5 mM DTT & 0.5 mM PMSF.
Preparation Method Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A, and then centrifugated at 25,000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C, and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged, divided into aliquots, and stored at -80°C. The protein concentration was determined by the method of Bradford (Bio-Rad protein assay, microplate standard assay). The lysate was adjusted to 2 mg/ml.
Quality Control Testing 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity 50 μg
Storage Buffer In Buffer D.
Host Species Human
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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