TdB Labs AB 500 kDa FITC-lysine-dextran Green Fluorophore

FITC-lysine-dextran is a derivative carrying both free primary amines and free carboxylates as well as the fluorescent dye Fluorescein (FITC). Lysine is a naturally occurring amino acid and a popular moiety for conjugation and fixation in biochemistry and life sciences. After purification, all the batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. FITC-lysine-dextrans are supplied by TdB Labs as a yellow to orange powder with molecular weights from 4 kDa to 500 kDa.

Our high quality FITC-lysine-dextran offer:

• Excellent molecular weight distribution (low polydispersity)
• Bright fluorescence
• Fixability in cells and tissue
• Easy conjugation to dyes or biomolecules
• Fully water soluble and excellent stability in solution or on the shelf

Structure

FITC-lysine-dextrans are synthesised from well-characterised dextran fractions derived from Leuconostoc mesenteroides. The dextran fractions are first labelled with lysine and thereafter functionalised with FITC under mild conditions to form FITC-lysine-dextran. The degree of substitution of lysine is 0.005 to 0.03 (mol lysine/mol glucose) and the degree of substitution of FITC is 0.001 to 0.01 (mol FITC/mol glucose).

Spectral data

The excitation maximum for FITC-lysine-dextran is 490 to 495 nm and the emission maximum is 520 ± 5 nm.

Storage and stability

FITC-lysine-dextran is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature.

Solubility

FITC-lysine-dextran dissolves readily in water.

Application

FlTC-lysine-dextran is a versatile tool for bioconjugation and fixation in living systems.