missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Biochemical Reagents
  4. Carbohydrates
  5. Glycosyl compounds
  6. 5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside, MP Biomedicals™

5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside, MP Biomedicals™

Product Code. 11486392 Shop All MP Biomedicals Products
Click to view available options
Quantity:
250 mg
1 g
Unit Size:
1g
250mg
This item is not returnable. View return policy

Product Code. 11486392

Brand: MP Biomedicals 0215000183

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Commonly known as X-Gal. 5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside, MP Biomedicals is a histochemical substrate for β-galactosidase. It is particularly useful in molecular biology, where β-galactosidase is frequently used as a report

5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques.

It is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

  • Insertion of DNA into the lac Z region results in the loss of β-galactosidase activity
  • Lac+ bacterial colonies resulting from a-complementation will appear blue
  • Bacterial colonies containing plasmid with DNA inserted in the lac Z region will be incapable of a-complementation and will appear white
TRUSTED_SUSTAINABILITY

Chemische Identifikatoren

CAS 7240-90-6
Molecular Formula C14H15BrClNO6
Molecular Weight (g/mol) 408.629
InChI Key OPIFSICVWOWJMJ-AEOCFKNESA-N
Synonym x-gal, 5-bromo-4-chloro-3-beta-d-galactopyranosyloxy indole, 5-bromo-4-chloro-3-indolyl-beta-d-galactoside, unii-v595og374w, 5-bromo-4-chloro-3-indolyl beta-galactoside, 5-bromo-4-chloroindol-3-yl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactoside, 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactopyranoside, indoxyl-gal
PubChem CID 65181
ChEBI CHEBI:75055
IUPAC Name (2S,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
SMILES C1=CC(=C(C2=C1NC=C2OC3C(C(C(C(O3)CO)O)O)O)Cl)Br

Spezifikation

Melting Point 230°C (decomposition)(literature)
Quantity 250 mg
Solubility Information Soluble in DMF (2% w/v, clear and colorless solution).
Formula Weight 408.6
Physical Form Powder
Berichtigung von Produktinhalten

Bitte geben Sie uns Ihr Feedback zu den Produktinhalten, indem Sie das folgende Formular ausfüllen.

Name des Produkts

Indem Sie auf Absenden klicken, erklären Sie sich damit einverstanden, dass Fisher Scientific sich mit Ihnen in Verbindung setzen kann, um Ihr Feedback in diesem Formular zu bearbeiten. Wir werden Ihre Informationen nicht für andere Zwecke weitergeben. Alle bereitgestellten Kontaktinformationen werden in Übereinstimmung mit unserer Datenschutzrichtlinie aufbewahrt. Datenschutzrichtlinie.

Vielen Dank, dass Sie uns helfen, unsere Website zu verbessern. Ihr Feedback wurde übermittelt