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  8. 3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide, MP Biomedicals

3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide, MP Biomedicals

Product Code. 11379079 Shop All MP Biomedicals Products
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Product Code. 11379079

Brand: MP Biomedicals 02102227.1

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Description

  • Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation
  • MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells
  • The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm
  • MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD
  • ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used
  • MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems

TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 298-93-1
Molecular Formula C18H16BrN5S
Molecular Weight (g/mol) 414.33
MDL Number MFCD00011964,MFCD00066662
InChI Key AZKSAVLVSZKNRD-UHFFFAOYSA-M
Synonym thiazolyl blue, thiazolyl blue tetrazolium bromide, mmt tetrazolium, methylthiazoletetrazolium, mtt, thiazolyl blue monotetrazolium, thiazole blue, 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide, mtt van, unii-euy85h477i
PubChem CID 64965
ChEBI CHEBI:53233
IUPAC Name 2-(4,5-dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-3H-1,2λ⁵,3,4-tetrazol-2-ylium bromide
SMILES [Br-].CC1=C(C)N=C(S1)[N+]1=NC(=NN1C1=CC=CC=C1)C1=CC=CC=C1

Specifications

Melting Point 189°C to 199°C
Color Yellow
Linear Formula C18H16BrN5S
Quantity 100 mg
Solubility Information Soluble in methyl alcohol (heat), methanol (10mg/mL with heat - clear, yellow solution)and water (5mg/mL-clear to very slightly hazy, yellow to bright yellow solution).
Formula Weight 414.3
Percent Purity ∼98%
Physical Form Powder
Chemical Name or Material 3-(4,5-DIMETHYLTHIAZOLYL-2)-2,5-DIPHENYL TETRAZOLIUM BROMIDE
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