Primary Antibodies
Browse through a wide range of primary antibodies that bind to specific proteins or biomolecules and are suitable for various research disciplines and applications. Choose from monoclonals for high reproducibility, polyclonals for quicker binding and affinity and conjugated or unconjugated species, as well as different forms, isotypes, and target species depending on your needs.
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FAQ
A primary antibody is the antibody that specifically binds to the antigen of interest in an experiment. It directly interacts with the target protein, peptide, or other molecule you are studying. The primary antibody's role is to recognize and bind to a specific epitope on the antigen, allowing for subsequent detection or measurement in various assays, such as Western blotting, immunohistochemistry, or ELISA.
To choose primary antibodies consider the following:
Specificity: Ensure it binds specifically to your target antigen.
Validation: Check if it's validated for your application.
Host Species: Use an antibody from a different species than your sample.
Antigen Source: Match the antibody's antigen source with your target.
Isotype: Consider the antibody's isotype for secondary antibody compatibility.
Literature: Review studies that used the antibody successfully.
Controls: Use appropriate controls to validate performance.
Supplier Reputation: Choose from reputable suppliers.
Class 1 and Class 2 antibodies typically refer to antibodies that react with Major Histocompatibility Complex (MHC) molecules:
Class I MHC Antibodies: Target MHC Class I molecules, which are present on almost all nucleated cells and present endogenous peptides to cytotoxic T cells (CD8+).
Class II MHC Antibodies: Target MHC Class II molecules, found on antigen-presenting cells (APCs) and present exogenous peptides to helper T cells (CD4+).
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