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Gibco™ Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation
Dynabeads Human T-Activator CD3/CD28 beads are 4.5-μm superparamagnetic beads covalently coupled with an optimized combination of anti-human CD3 and anti-human CD28 antibodies and intended for ex vivo activation and expansion/proliferation of human T cells.
| Shipping Condition | Ambient Temperature |
|---|---|
| Clonality | Monoclonal |
| Target Species | Human T-cells |
| Host Species | Mouse |
| Surface Functionality | Epoxy |
| Isolation Technology | Magnetic Beads |
| Material | Magnetic Polystyrene |
| Concentration | 4 x 10 7 Beads/mL |
| For Use With (Application) | Activation and Expansion of T-cells |
| Regulatory Status | For Research Use Only |
| Reactivity | Human |
| For Use With (Equipment) | DynaMag™ Magnet |
| Product Type | Antibody Coated Bead |
| Diameter (Metric) | 4.5 μm |
| Shelf Life | 24 months from date of manufacture |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Uniformity | Monosized 4.5 μm (CV <5%) |
| Product Line | DynaBeads |
Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.
| Buffer | Lysis Buffer |
|---|---|
| Content And Storage | Upon receipt store at 4°C |
| Inhibitors | Protease Inhibitor, Phosphatase Inhibitor |
| Form | Liquid |
| Product Type | Protease and Phosphatase Inhibitor Cocktail |
| Reagent Type | Protease Inhibitor, Phosphatase Inhibitor |
| Product Line | Halt |
Thermo Scientific™ Lysozyme Solution (50 mg/mL)
Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.
Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail
Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.
| Content And Storage | Upon receipt store product at 4°C. Do not freeze. |
|---|---|
| Inhibitors | Phosphatase Inhibitor |
| Form | Liquid |
| Product Type | Phosphatase Inhibitor Cocktail |
| Reagent Type | Phosphatase Inhibitor |
| Product Line | Halt |
Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
| Buffer | Lysis Buffer |
|---|---|
| Content And Storage | Upon receipt store at 4°C |
| Inhibitors | Protease Inhibitor |
| Form | Liquid |
| Product Type | Protease Inhibitor Cocktail |
| Reagent Type | Protease Inhibitor |
| Product Line | Halt |
Thermo Scientific™ Brij™-35, 30% Solution
Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.
Thermo Scientific™ Pierce™ High Capacity Streptavidin Agarose
Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.
| Particle Size | 45 to 165 μm |
|---|---|
| Stationary Phase | Streptavidin |
| Type | Agarose |
| Target | Biotin-Tagged Molecule |
| Content And Storage | Upon receipt store at 4°C. Do not freeze the resin. Product is shipped at ambient temperature. |
| Format | Bottle |
| Protein Form | Recombinant |
| Column Type | Affinity, Agarose Resin |
| Sufficient For | Binding ≥/= 100 μg Biotin/mL Resin (≥/= 10 mg Biotinylated BSA/mL Resin) |
| Product Line | Pierce |
Thermo Scientific™ M-PER™ Mammalian Protein Extraction Reagent
Isolate total protein from cultured mammalian cells with this highly efficient lysis and extraction reagent.
| Content And Storage | Upon receipt store product at room temperature. |
|---|---|
| Format | Liquid |
| Product Type | Protein Extraction Reagent |
| Product Line | M-PER |
Thermo Scientific™ Pierce™ Anti-HA Magnetic Beads
Perform immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.
| Particle Size | 1 μm |
|---|---|
| Ligand Type | Antibody |
| Type | Magnetic Affinity Bead |
| Target | HA |
| Content And Storage | Store at 4°C. |
| Protein Form | Recombinant |
| Form | Liquid |
| Concentration | Slurry: 10mg/mL, 1% solids |
| Product Line | Pierce |
Thermo Scientific™ Pierce™ Centrifuge Columns
Pack these empty 0.5 to 10 mL spin columns with agarose beads to perform small-scale affinity purification methods in 15- or 50-mL conical microcentrifuge tubes.
| Content And Storage | Store at room temperature. |
|---|---|
| Column Type | Empty Column, Polyethylene Filter |
| Product Line | Pierce |
Invitrogen™ Dynabeads™ Protein G Immunoprecipitation Kit
Dynabeads Protein G Immunoprecipitation Kit is intended for immunoprecipitation (IP) and includes all reagents and buffers required to perform IP using your own antibody.
Thermo Scientific™ Detergent Removal Spin Columns & Plates
Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.
| Stationary Phase | Proprietary Ligand |
|---|---|
| Column Type | Proprietary Resin, Affinity |
| Purification Target | Detergent Removal |
Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Spin Columns, 1 mL
Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.
| Stationary Phase | Proprietary Ligand |
|---|---|
| Type | Spin Column |
| Target | Endotoxin Removal |
| Content And Storage | Upon receipt store at 4°C. |
| Format | Spin Column |
| Form | Liquid |
| Column Type | Affinity, Cellulose Resin |
| Formulation | 22mL-capacity centrifuge columns containing 1mL resin |
| Capacity (Metric) | Up to 2,000,000 EU/mL |
| Product Line | Pierce |
Thermo Scientific™ Pierce™ Protein G Magnetic Beads
Perform antibody purification and immunoprecipitation methods using manual or robotic magnetic separators with these high-capacity and high-throughput affinity particles.
| Particle Size | 1 μm |
|---|---|
| Ligand Type | Protein G |
| Type | Magnetic Affinity Bead |
| Target | Antibody |
| Content And Storage | Upon receipt store at 4°C. Product shipped with an ice pack. |
| Protein Form | Recombinant |
| Form | Liquid |
| Concentration | Slurry: 10mg/mL, 1% solids |
| Product Line | Pierce |