Classical and Basal Cell Culture Media
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Gibco™ Ham's F-12K (Kaighn's) Medium
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Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Storage conditions: 2-8°C. Protect from light Shipping conditions: Ambient |
| Form | Liquid |
| Without Additives | No HEPES |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Concentration | 1 X |
| Sterility | Sterile-filtered |
| Cell Type | Human Hepatocytes |
| Sterilization Method | Sterile-filtered |
| With Additives | Low Glucose,Glutamine,Phenol Red,Sodium Pyruvate |
| Product Type | Ham's F-12K (Kaighn's) Medium |
| Classification | Animal Origin-free |
| Shelf Life | 12 Months From Date of Manufacture |
| Product Line | Gibco |
Gibco™ MEM, no glutamine, no phenol red
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MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media.
Gibco™ Ham's F-12 Nutrient Mix
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Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells.
| Cell Line | CHO, COS-7, and rat prostate epithelial cells |
|---|---|
| Sterilization Method | Sterile-filtered |
| With Additives | Glutamine,Phenol Red,Sodium Pyruvate |
| Product Type | Ham's F-12 Nutrient Mixture |
| Form | Liquid |
| Without Additives | No HEPES |
| Classification | Animal Origin-free |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Shelf Life | 12 Months From Date of Manufacture |
| Concentration | 1 X |
| Sterility | Sterile-filtered |
| Product Line | Gibco |
| Cell Type | Primary Rat Astrocytes |
Gibco™ Advanced MEM
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Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
| Shipping Condition | Ambient |
|---|---|
| Cell Line | HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts |
| Content And Storage | Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Form | Liquid |
| Without Additives | No Glutamine,No HEPES |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Concentration | 1 X |
| Sterility | Sterile-filtered |
| Sterilization Method | Sterile-filtered |
| With Additives | Low Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA) |
| Product Type | MEM (Minimum Essential Medium) |
| Classification | Animal Origin |
| Shelf Life | 12 Months From Date of Manufacture |
| Product Line | Gibco |
| Serum Level | Reduced Serum |
Gibco™ BenchStable™ RPMI 1640
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Gibco RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer.
| Shipping Condition | Room Temperature |
|---|---|
| Cell Line | HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas |
| Content And Storage | Store at room temperature. Protect from light. |
| Form | Liquid |
| Without Additives | No HEPES,No Sodium Pyruvate |
| Manufacturing Quality | cGMP-compliant under the ISO 9001: 2015 standard |
| Green Features | ACT Ecolabel (Silver) |
| Concentration | 1 X |
| Sterility | Sterile-filtered |
| With Additives | GlutaMAX,Phenol Red,Phenol Red |
| Product Type | RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) |
| Classification | Animal Origin-free |
| Shelf Life | 12 Months From Date of Manufacture |
| Product Line | BenchStable |
| Serum Level | Standard Serum Supplementation |
Gibco™ CTS™ OpTmizer™ T Cell Expansion SFM, bag format
CTS™ OpTmizer™ T Cell Expansion SFM, bag format
Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement
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RPMI 1640 Medium, GlutaMAX™ Supplement
| Content And Storage | 2 to 8°C |
|---|---|
| With Additives | GlutaMAX,Phenol Red |
| Product Type | Roswell Park Memorial Institute (RPMI) 1640 Medium |
| Form | Liquid |
| Without Additives | No HEPES,No Sodium Pyruvate |
| pH | 7.0 to 7.4 |
| Certifications/Compliance | ISO 13485 standards, FDA |
| Shelf Life | 12 Months |
| Concentration | 1 X |
| For Use With (Application) | Mammalian cell culture |
Gibco™ KnockOut™ DMEM
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KnockOut D-MEM is a basal medium optimized for growth of undifferentiated embryonic and induced pluripotent stem cells. The osmolarity is optimized to approximate that of mouse embryonic tissue.
Gibco™ KnockOut™ DMEM/F-12
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KnockOut™ DMEM/F-12
Gibco™ CD 293 Medium (1X)
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CD 293 Medium is a chemically-defined, protein-free medium optimized for the growth of suspension cultures of 293 cells. CD 293 Medium contains no proteins or peptides of animal, plant, or synthetic origin.
Gibco™ FreeStyle™ F17 Expression Medium
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An animal origin-free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation.
| Cell Line | HEK293 |
|---|---|
| Culture Environment | CO2 |
| Purity or Quality Grade | Qualified for Further Manufacturing |
| Form | Liquid |
| Without Additives | No Glutamine |
| Endotoxin Level | < 1 EU/mL |
| Concentration | 1 X |
| For Use With (Application) | Protein expression, viral vector production |
| Sterility | Sterile |
| Product Type | Expression Medium |
| Classification | Animal Origin-free,Chemically-defined,Protein-free,Serum-free |
| Culture Type | Suspension Cell Culture |
| Product Line | Freestyle |
| Serum Level | Serum-free |
Gibco™ Leibovitz's L-15 Medium
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Leibovitz's L-15 Medium
| Regulatory Status | For in-vitro diagnostic use |
|---|---|
| Content And Storage | 2 to 8°C |
| Description | Leibovitz's L-15 Medium, 500mL, 1 X Concentration, Liquid Form, With Galactose, Phenol Red, L-glutamine, Sodium Pyruvate, 7.4 to 7.9 pH, 12 Months Shelf Life, 300 to 340mOsm/kg Osmolality, Used for Mammalian Cell Culture |
| With Additives | Galactose,Phenol Red,Glutamine,Sodium Pyruvate |
| Product Type | Leibovitz's L-15 Medium |
| Form | Liquid |
| Without Additives | No Glucose,No HEPES,No Sodium Bicarbonate |
| pH | 7.4 to 7.9 |
| Certifications/Compliance | ISO 13485 standards, FDA |
| Shelf Life | 12 Months |
| Concentration | 1 X |
| For Use With (Application) | Mammalian cell culture |
Gibco™ RPMI 1640 Medium
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RPMI 1640 Medium
| Regulatory Status | For in-vitro diagnostic use |
|---|---|
| Product Type | RPMI 1640 Medium |
| Form | Liquid |
| Certifications/Compliance | ISO 13485 standards, FDA |
| For Use With (Application) | Mammalian cell culture |
Gibco™ MEM α, nucleosides
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MEM α, nucleosides
| Regulatory Status | For in-vitro diagnostic use |
|---|---|
| Content And Storage | 2 to 8°C |
| Description | MEM α± Medium, 500mL, 1 X Concentration, Liquid Form, With Ribonucleosides, Deoxyribonucleosides, L-glutamine, Phenol Red, Sodium Pyruvate, 7 to 7.4 pH, 12 Months Shelf Life, 280 to 310mOsm/kg Osmolality, Used for Mammalian Cell Culture |
| With Additives | Glutamine,Phenol Red,Sodium Pyruvate,Ribonucleosides,Deoxyribonucleosides |
| Product Type | MEM α± Medium |
| Form | Liquid |
| pH | 7 to 7.4 |
| Certifications/Compliance | ISO 13485 standards, FDA |
| Shelf Life | 12 Months |
| Concentration | 1 X |
| For Use With (Application) | Mammalian cell culture |
Gibco™ DMEM, high glucose, no glutamine, no methionine, no cystine
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Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.