Protein Labeling Reagents
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Cytiva CypHer5E NHS Esters
A red-excitable, pH-sensitive cyanine dye derivative, reporting receptors movement from the cell surface into acidic endosomes upon agonist stimulation.
| Product Type | CypHer5E NHS Ester |
|---|---|
| Formulation | 100% CypHer 5E |
| Color | Blue |
| Solubility | Easily soluble in methanol; Very slightly soluble in cold water and hot water. |
| Emission | 663 |
| For Use With (Application) | This reagent has been shown to be suitable for live cell receptor internalization studies using the IN Cell Analyzer 3000, IN Cell Analyzer 1000, and confocal microscopes. 0.5 mL of stock reagent will be sufficient for up to 500 wells. |
Cytiva DeStreak Rehydration Solution
Highly suitable for both analytical and preparative gels. DeStreak Rehydration Solution improves reproducibility and quality of 2-D gels by preventing streaking.
| Content And Storage | 2°C to 8°C |
|---|---|
| Kit Contents | Optimized concentrations of urea, thiourea, CHAPS and Destreak reagent |
| pH Range | >7 |
| Product Type | Destreak Reagent |
| For Use With (Application) | Improves reproducibility and quality of 2D Gels by preventing streaking |
Cytiva Cy5.5 NHS Ester Mono-Reactive CyDye
Use for highly accurate labeling of amine groups. Cytiva Cy5.5 NHS Ester Mono-Reactive CyDye contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides.
| Kit Contents | Foil packs of premeasured dye |
|---|---|
| Format | Powdered |
| pH Range | 3 to 10 |
| Product Type | Cy5.5 NHS Ester |
| Color | Blue |
| Label or Dye | Cy5.5 |
Cytiva Amersham™ QuickStain Protein Labeling Kit
Amersham QuickStain enables protein detection directly after SDS-PAGE or transfer. Supports Western blot normalization for quantitative Western blotting.
Cytiva Cy3 NHS Ester Mono-Reactive CyDye
Use for highly accurate labeling of amine groups. Cytiva Cy3 NHS Ester Mono-Reactive CyDye contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides.
| Kit Contents | Foil packs of pre-measured dried dye |
|---|---|
| Format | Dried |
| pH Range | 3 to 10 |
| Product Type | Cy3 NHS Ester |
| Color | Red |
| Label or Dye | Cy3 |
Cytiva Amersham™ Streptavidin-Fluor Conjugates
Use for in situ hybridization and flow cytometry. Cytiva Life Sciences™ Amersham™ Streptavidin-Fluor Conjugates consist of streptavidin conjugated to a range of fluorescent dyes.
| Formulation | This product is supplied as a lyophilized solid in Phosphate-buffered Saline (0.02M Potassium Phosphate, 0.15M NaCl) pH 7.2, containing 1%(w/v) Bovine Serum Albumin and 0.1% (w/v) Sodium Azide, reconstitute with 1mL of deionized Water, dispense into suitable aliquots and freeze, avoid repeated freeze-thaw cycles |
|---|---|
| Quantity | 1 mg |
| For Use With (Application) | Suitable for use in the Fluorescent analysis of Biotinylated samples |
Cytiva CyDye™ DIGE Fluors: Cy3
Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel
| Content And Storage | -15°C to -30°C |
|---|---|
| Format | Solid |
| Product Type | Amersham CyDye DIGE Fluor Cy3 Minimal Dye |
| Color | Green |
| For Use With (Application) | For general 2-D applications where sufficient amounts of sample are available, detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel |
Cytiva CyDye™ DIGE Fluors: Cy5
Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel
| Content And Storage | -15°C to -30°C |
|---|---|
| Format | Solid |
| Product Type | Amersham CyDye DIGE Fluor Cy5 Minimal Dye |
| Color | Red |
| For Use With (Application) | For general 2-D applications where sufficient amounts of sample are available, detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel |
Cytiva CyDye™ DIGE Fluor Minimal Dye Labeling Kits
Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel. Amersham CyDye™ DIGE Fluor Minimal Labeling Kits contain size- and charge-matched fluorescent dyes specifically designed for detecting protein abundance differences in 2-D Fluorescence Difference Gel Electrophoresis using Ettan DIGE system.
| Content And Storage | -15°C to -30°C |
|---|---|
| Format | Solid |
| Color | Blue, Green, Red |
Cytiva CyDye™ DIFluors: Saturation Dye Labeling Kits
Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel
| Content And Storage | -15°C to -30°C |
|---|---|
| Format | Solid |
| Product Type | Amersham CyDye DIGE Fluor Labeling Kit |
| Color | Green, Red |
Cytiva CyDye™ DIGE Fluors: Cy2
Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel
| Content And Storage | -15°C to -30°C |
|---|---|
| Format | Solid |
| Product Type | Amersham CyDye DIGE Fluor Cy2 Minimal Dye |
| Color | Blue |
| For Use With (Application) | For general 2-D applications where sufficient amounts of sample are available, detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel |
Cytiva Amersham™ CyDye™ Reactive Dye 5-Packs
Use for the most accurate labeling. Cytiva Amersham™ CyDye™ Reactive Dye 5-Packs contain derivatized CyDye that carries only one reactive group on each dye molecule for accurate labeling of amine groups.
| Kit Contents | Five foil packs each containing dried dye to label 1mg of Protein |
|---|---|
| Sufficient For | Labeling 1mg Protein or 30nM Amino groups |
| Quantity | Each |
Cytiva Amersham™ CyDye™ Value Packs (mono-Reactive Hydrazide)
For the labeling of free carbonyl groups of glycoproteins and carbohydrates
| Content And Storage | 2°C to 8°C, protected from light. Reconstituted material may be stored for up to 2 weeks at -20°C in aliquots to avoid repeat freeze thaw cycles. |
|---|---|
| pH Range | 3 to 10 |
| Solubility | Soluble in cold water and hot water |
| For Use With (Application) | For labelling via carbonyl groups. For use in steady state fluorescence, FP and FRET applications |
Cytiva Cy™3B Mono-reactive NHS Ester Fluorescent Dyes
Combines exceptional photostability with bright and intense signals. Cytiva Cy™3B NHS Ester Mono-Reactive CyDye™ provides targeted and specific labeling of amine residues on antibodies, peptides, or oligonucleotides in fluorescence polarization applications.
| Format | Solid |
|---|---|
| Product Type | Cy3B NHS Ester |
| Molecular Weight (g/mol) | 771 |
| Formulation | 100% Cy3B (NHS ester) |
| Color | Red |
| Solubility | Soluble in aqueous buffers |
| Emission | 570 |
| Purity | >99% |
| Excitation | 559 |
Cytiva Amersham™ CyDye™ Value Packs (mono-Reactive Maleimide)
For the selective labeling of free sulfhydryl groups such as cysteine residues in peptides, proteins, and oligonucleotides
| Kit Contents | Foil packs, each containing 1, 5 or 25mg dried dye |
|---|---|
| pH Range | 3 to 10 |