Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100 to 200 μg/mL.
Perform DNA extractions with this phenol:chloroform:isoamyl alcohol mixture (25:24:1), accompanied by a buffer to equilibrate the solution at either pH 6.7 or pH 8.0.
Purified Torulla Invitrogen yeast RNA is suitable as a coprecipitant in nucleic acid precipitations. It cannot be used in reactions inhibited by exogenous RNA, and it is the most inexpensive source of a high-quality coprecipitant.
Sodium Phosphate Buffer is a reagent with very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. It occurs in several forms: monobasic (NaH2PO4), dibasic (Na2HPO4), and tribasic (Na3PO4).
UltraPure Phenol is used in the purification of nucleic acids. In a mixture of phenol and buffered aqueous solution, proteins are denatured and collect at the interphase while most nucleic acids remain in the aqueous phase.