Direct PCR Reagents and Kits
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Thermo Scientific™ Phire Plant Direct PCR Master Mix
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Phire Hot Start II DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Master Mix |
| Sample Type | Plant |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| Starting Material | DNA |
| For Use With (Application) | Genotyping,Hot-start PCR |
| PCR Method | Direct PCR |
Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| GC-Rich PCR Performance | Medium |
| Includes Label or Dye | Yes |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Genotyping,Hot-start PCR |
| PCR Method | Direct PCR |
| Polymerase | Phire Hot Start II DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Master Mix |
| Sample Type | Tissue |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Invitrogen™ Fast SYBR™ Green Cells-to-CT™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Ambion™ Fast SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | Store Stop Solution, DNase I and 20X RT Enzyme Mix at -20°C. Store Lysis Solution, 2X SYBR™ RT Buffer, Fast SYBR™ Green PCR Master Mix at 4°C. Store Nuclease-free Water at room temperature. |
| Purity or Quality Grade | UP (Ultra Pure) |
| Reverse Transcriptase | M-MLV |
| Purification Time | 5 min. |
| Green Features | Fewer resources used and less waste, Less hazardous |
| Concentration | 20X |
| For Use With (Application) | RT-PCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Fast DNA Polymerase |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
| Sample Type | Cells |
| Reaction Speed | Fast |
| Label or Dye | SYBR Green |
| Hot Start | Built-In Hot Start |
| Product Line | Cells-to-CT, SYBR |
Invitrogen™ Cells-to-cDNA™ II Kit
The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature. |
| Reverse Transcriptase | M-MLV |
| Size (Final Product) | 7 kb or less |
| Final Product Type | cDNA |
| Sample Type | Mammalian Cells |
| Purification Time | 2 hr. |
| Optimal Reaction Temperature | 42°C |
| For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
| Product Line | Ambion, Cells-to-cDNA |
Thermo Scientific™ Phusion Blood Direct PCR Master Mix
Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Includes Label or Dye | Yes |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Genotyping |
| PCR Method | Direct PCR |
| Polymerase | Phusion Blood II DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Master Mix |
| Sample Type | Blood |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Ambion™ Power SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.
| Shipping Condition | Dry Ice |
|---|---|
| Purity or Quality Grade | LD (Low DNA) |
| Reverse Transcriptase | M-MLV |
| Purification Time | 5 min. |
| Green Features | Fewer resources used and less waste, Less hazardous |
| Concentration | 20X |
| For Use With (Application) | RT-PCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Sample Type | Cells |
| Reaction Speed | Standard |
| Label or Dye | SYBR Green |
| Hot Start | Built-In Hot Start |
| Product Line | Cells-to-CT, SYBR |
Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit
Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.
Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit
Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.
Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit (with competent cells)
Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.
Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells)
Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.