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Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit (with competent cells)

Product Code. 15473903
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10 reactions
Unit Size:
10 reactions
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For research use only. Not for use in diagnostic procedures.
Product Code. 15473903 Supplier Invitrogen™ Supplier No. A21178

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This item is not returnable. View return policy
For research use only. Not for use in diagnostic procedures.

Description

Includes

  • CRISPR OFP Nuclease Vector, linearized
  • 10X Oligonucleotide Annealing Buffer
  • DNase/RNase-free Water
  • 5X Ligation Buffer
  • T4 DNA Ligase
  • U6 Forward Sequencing Primer
  • Control, double stranded oligonucleotide
  • OneShot(TM) TOP10 Chemically Competent Cells

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.

Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter.

GeneArt CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. The OFP reporter allows for fluorescence-based tracking of transfection efficiency, as well as FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells.

The linearized GeneArt™ CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. Competent cells included for added convenience and highest cloning efficiencies.

GeneArt™ CRISPR Nuclease Vector system offers:
  • Easy-to-design genome engineering system
  • Affordable, ready-to-use cloning vectors
  • Enrichment for hard to transfect or difficult to modify cell
For research use only. Not for use in diagnostic procedures.

Certifications

CoA

Compliance

CoA

Recommended Storage

Store in freezer (-5 to -30°C)

TRUSTED_SUSTAINABILITY

Specifications

Cloning Method Restriction Enzyme/MCS
Content And Storage • CRISPR OFP Nuclease Vector, linearized
• 10X Oligonucleotide Annealing Buffer
• DNase/RNase-free Water
• 5X Ligation Buffer
• T4 DNA Ligase
• U6 Forward Sequencing Primer
• Control, double stranded oligonucleotide
Store the above at -5 to -30°C.

• OneShot™ TOP10 Chemically Competent Cells
Store at -80°C.
Detection Method Primer-probe
Form Liquid
Format Kit
Reaction Speed Fast
Technique CRISPR-Cas9
Product Line GeneArt
Product Type Crispr Nuclease Vector With ofP Reporter Kit
Promoter U6, CMV
Protein Tag OFP fusion
Quantity 10 reactions
Reporter Gene OFP
Sufficient For 10 Reactions
For Use With (Application) Gene Expression, Cloning
No. of Reactions 10 Reactions
Overhang 3' overhangs
Starting Material Oligos (DNA)
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For Research Use Only. Not for use in diagnostic procedures.

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