PCR Supplies
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Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Kit |
| Reverse Transcriptase | SuperScript IV |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Quantity | Each |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Applied Biosystems™ TaqMan™ Universal PCR Master Mix
TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.
| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Product Type | Universal PCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
| PCR Method | qPCR |
| Product Line | TaqMan™ |
| Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0
Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.
| Purity or Quality Grade | UP (Ultra Pure) |
|---|---|
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Genotyping,Pathogen Detection,Virus Detection |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Format | Bottle |
| Product Type | qPCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode |
| Sample Type | Environmental Sample, Food |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Product Line | TaqMan™ |
Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | Maxima H Minus |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 20 kb |
| Reaction Format | Separate components |
| Reaction Speed | 15 to 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C to 55°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
TaqMan™ Advanced miRNA cDNA Synthesis Kit
Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.
Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR
TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.
| Shipping Condition | Dry Ice |
|---|---|
| Reverse Transcriptase | M-MLV |
| Form | Liquid |
| InhibitorToleranceSimple | High |
| For Use With (Application) | Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 3-plex |
| Detection Method | Primer-probe |
| Thermostability | Not Applicable |
| Polymerase | AmpliTaq Fast DNA Polymerase |
| Format | Tube |
| Product Type | 1-Step Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System |
| Sample Type | Total RNA, Viral DNA, Viral RNA, mRNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | RNA only, Both RNA and DNA |
| Hot Start | Built-In Hot Start |
| Product Line | TaqMan™ |
Invitrogen™ Platinum™ SuperFi II PCR Master Mixes
Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion U Hot Start PCR Master Mix
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion U Hot Start DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Content And Storage | • Phusion U Hot Start DNA Polymerase • 2X reaction buffer • 3 mM MgCl2 • 400 μM each of dATP, dTTP, dCTP and dGTP Master mix is supplied with nuclease-free water. Store at -20°C. |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR |
Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)
Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.
| Polymerase | DreamTaq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Reaction Speed | Standard |
| Hot Start | No |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Standard PCR,High-fidelity PCR |
Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)
Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.
| GC-Rich PCR Performance | High |
|---|---|
| Fidelity (vs. Taq) | 1X |
| Format | Tube |
| Size (Final Product) | 5 kb or less |
| Hot Start | Built-In Hot Start |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR |
DreamTaq™ Hot Start PCR Master Mixes
Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | DreamTaq DNA Polymerase |
| GC-Rich PCR Performance | Low |
| Reaction Format | SuperMix or Master Mix |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Overhang | 3'-A |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR |
Thermo Scientific™ Phusion™ Plus PCR Master Mixes
Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Ready to use master mix |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
| Shipping Condition | Wet Ice |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Reverse Transcriptase | ArrayScript™ UP |
| For Use With (Application) | Gene Expression |
| PCR Method | 1-step RT-qPCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System |
| Sample Type | RNA |
| Reaction Speed | Standard |
| Label or Dye | SYBR Green |
| Run Time | Standard |
| Product Line | RNA-to-CT™, SYBR™ |
Thermo Scientific™ Phusion Hot Start II High-Fidelity PCR Master Mixes
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Hot Start II High-Fidelity DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |