accessibility menu, dialog, popup

UserName

Western Blot Substrates and Substrate Kits

Chemiluminescent substrates and chromogenic precipitating substrates for western blot detection of alkaline phosphatase (AP) and horseradish peroxidase (HRP) enzyme conjugates; includes both routine and high-sensitivity detection materials.
Quantity 
  • (1)
  • (1)
  • (1)
  • (3)
  • (1)
  • (10)
  • (2)
  • (2)
  • (1)
  • (1)
  • (1)
  • (1)
  • (1)
  • (4)
  • (1)
  • (8)
  • (1)
  • (1)
  • (1)
  • (2)
  • (7)
  • (2)
  • (1)
  • (1)
  • (1)
  • (1)
  • (1)
  • (1)
  • (10)
  • (1)
  • (1)
  • (1)
  • (1)
Product Type 
  • (1)
  • (1)
  • (1)
  • (6)
  • (3)
  • (4)
  • (2)
  • (36)
  • (1)
  • (1)
For Use With (Application) 
  • (6)
  • (1)
  • (1)
  • (2)
  • (1)
  • (2)
  • (34)
Detection Method 
  • (28)
  • (2)
  • (1)
  • (11)
Substrate 
  • (5)
  • (32)
  • (2)

Filtered Search Results

Products from some of our suppliers do not display in filtered search results. Please clear all filters to see these products.

Narrow Results

Narrow Results

No results found within this category. Try removing some selected filters and try again.

category

special offers

  • (21)
  • (9)

brands

  • (1)
  • (7)
  • (2)
  • (4)
  • (11)
  • (2)
  • (3)
  • (2)
  • (44)

special programs

  • (5)
  • (3)

Quantity

  • (1)
  • (1)
  • (1)
  • (3)
  • (1)
  • (10)
  • (2)
  • (2)
  • (1)
  • (1)
  • (1)
  • (1)
  • (1)
  • (4)
  • (1)
  • (8)
  • (1)
  • (1)
  • (1)
  • (2)
  • (7)
  • (2)
  • (1)
  • (1)
  • (1)
  • (1)
  • (1)
  • (1)
  • (10)
  • (1)
  • (1)
  • (1)
  • (1)

Product Type

  • (1)
  • (1)
  • (1)
  • (6)
  • (3)
  • (4)
  • (2)
  • (36)
  • (1)
  • (1)

For Use With (Application)

  • (6)
  • (1)
  • (1)
  • (2)
  • (1)
  • (2)
  • (34)

Detection Method

  • (28)
  • (2)
  • (1)
  • (11)

Substrate

  • (5)
  • (32)
  • (2)

Form

  • (35)
  • (3)

Product Line

  • (6)
  • (1)
  • (8)
  • (21)

Membrane Compatibility

  • (33)
1 – 15 of 46 results
1
Promotions Available

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

EDGE
Substrate Horseradish Peroxidase
Content And Storage Upon receipt store at 2-8°C. Product is shipped at ambient temperature.
Form Liquid
Product Type Substrate
Signal Duration 1 to 2 hr.
Product Line Pierce™
Detection Method Chemiluminescence
2

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

EDGE
Shipping Condition Room Temperature
Substrate Horseradish Peroxidase
Recommended Antibody Concentrations 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
Conjugate HRP
Form Liquid
Product Type Substrate
Signal Duration 6 to 24 hr.
For Use With (Application) Western Blot
Product Line SuperSignal™
Detection Method Chemiluminescence
3

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.

EDGE
4
Promotions Available

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Substrate Horseradish Peroxidase
Form Liquid
Product Type Substrate
Signal Duration 6 to 8 hr.
Product Line SuperSignal™
Detection Method Chemiluminescence
5

Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

EDGE
Sensitivity ∼1 pg
Product Type ECL Western Blot Detection Reagent
For Use With (Application) Sensitive Quantitative or Confirmatory Western Blot
6
Promotions Available

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Substrate Horseradish Peroxidase
Sensitivity Mid-femtogram
Form Liquid
Product Type Substrate
Signal Duration 24 hr.
Product Line SuperSignal™
Detection Method Chemiluminescence
7

Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Content And Storage Upon receipt store at 4°C. Product shipped on ice.
Sensitivity Low picogram to mid-femtogram
Form Liquid
Product Type Substrate
Membrane Compatibility Nitrocellulose, PVDF
Product Line Pierce™
Detection Method Chemiluminescence, Fluorescence
8
Promotions Available

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto

Contains SuperSignal™ West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.

EDGE
9
Promotions Available

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Pico PLUS + trial-size Atto

Contains SuperSignal™ West Pico PLUS Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate, providing chemiluminescent detection substrates for both routine and high sensitivity western blotting.

10
Promotions Available

Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate

SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.

Shipping Condition Wet Ice
Substrate Horseradish Peroxidase
Sensitivity Low-femtogram to high-attogram
Form Liquid
Product Type Substrate
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal™
Detection Method Chemiluminescence
11

Merck Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

Substrate Horseradish Peroxidase
Content And Storage 2°C to 8°C
Shelf Life 9 Months
For Use With (Application) Dot/Slot Blot
12

Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution

Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

13

Thermo Scientific™ SuperSignal™ Western Blot Substrate Trial Pack, Atto & Pico PLUS

Contains sample sizes of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate and SuperSignal™ West Pico PLUS Chemiluminescent Substrate to enable trial use of the most recommended substrates for routine and high-sensitivity western blotting.

14
Promotions Available

SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Pico PLUS

This substrate kit is comprised of SuperSignal West Femto Maximum Sensitivity Substrate plus a sample size of SuperSignal West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both high sensitivity and routine western blotting.

15
Promotions Available

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Atto + trial-size Pico PLUS

Contains SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both routine and high sensitivity western blotting.