Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
Filtered Search Results
| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 6.1% |
| Intraassay CV | 3.9 |
| Gene Symbol | GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1 |
| Total Assay Time | 2 hrs 30 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | <5 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 15.6 to 1000 pg/mL |
| For Use With (Application) | Colorimetric |
| Test Time | 1 hrs 20 mins |
| Accession Number | P10145 |
| Regulatory Status | RUO |
| Target | IL-8 |
| Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| Precision | <5 pg/mL |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3576 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions
Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
| Substrate Properties | Chemical Substrate |
|---|---|
| Product Type | ELISA Substrate |
| Substrate Type | HRP (Horseradish Peroxidase) |
| Detection Method | Colorimetric |
| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Description | Cortisol Competitive ELISA Kit |
| Interassay CV | 8.1% |
| Intraassay CV | 8.8 |
| Total Assay Time | 1 hrs 30 mins |
| Sample Volume | Fecal Extract 50 μL, Plasma 1 μL, Saliva 12.5 μL, Serum 1 μL, Supernatant 50 μL, Urine 6.25 μL |
| Assay Sensitivity | 17.3 pg/mL |
| Storage Requirements | -20°C |
| Research Discipline | Inflammation |
| Assay Range | 100 to 3200 pg/mL |
| For Use With (Application) | ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti |
| Test Time | 1 hrs 20 mins |
| Detection Method | Fluorescence |
| Regulatory Status | RUO |
| Target | Cortisol |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,HRP-Conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Sample Type | Fecal Extract,Plasma,Saliva,Serum,Supernatant,Urine |
| Label or Dye | HRP |
Gibco™ Residual Protein TrypLE™ ELISA Kit
The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLE enzyme.
| Shipping Condition | Wet Ice |
|---|---|
| Protein Aliases | TrypLE, R-Protease |
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Content And Storage | • Pre-coated 96-well plate • Standard assay buffer concentrate • HRP-conjugated detection antibody • Wash buffer concentrate • Chromogen stop solution • Adhesive plate covers Store at 2–8°C. |
| Conjugate | Horseradish Peroxidase (HRP) |
| Interassay CV | 6.20% |
| Intraassay CV | 7.20% |
| Total Assay Time | 1.5 hr. |
| Assay Sensitivity | 0.032 ng/mL |
| Assay Range | 0.78 to 50 ng/mL |
| Target | TrypLE (R-Protease) concentration |
| Format | Wash steps required |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Label or Dye | Horseradish Peroxidase (HRP) |
Invitrogen™ Cytokine 25-Plex Human Panel
Specifically designed for quantifying human cytokines and chemokines in serum, plasma, and tissue culture supernatant
Invitrogen™ Superoxide Dismutase (SOD) Colorimetric Activity Kit
Assay Superoxide Dismutase (SOD) Activity in Serum, Plasma, Cells, Tissues and Erythrocyte Lysates.
| Shipping Condition | Approved for shipment on Wet or Dry Ice |
|---|---|
| Immunoassay Kit Format | Assay Activity Kit |
| Content And Storage | 96-well plate(s), standard, detection reagent, assay buffer, substrate, and lot-specific technical data sheet. Store at –20°C. |
| For Use With (Equipment) | Microplate Reader |
| Target Kit Named | Superoxide Dismutase (SOD) |
Thermo Scientific™ CaptureSelect™ AAVX Ligand Leakage ELISA
Thermo Scientific™ CaptureSelect™ AAVX Ligand Leakage ELISA is designed to detect possible leached ligand of adeno-associated viruses (AAV) purified using POROS CaptureSelect AAVX Affinity Matrix.
| Shipping Condition | Dry Ice |
|---|---|
| Product Line | CaptureSelect |
Invitrogen™ Cell Lysis Buffer II
Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.
Thermo Scientific™ PNPP Substrates
Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.
| Substrate Properties | Chemical Substrate |
|---|---|
| Product Type | ELISA Substrate |
| Substrate Type | Alkaline Phosphatase |
| Detection Method | Colorimetric |
Thermo Scientific™ 1-Step™ ABTS Substrate Solution
Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.
| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 6.6% |
| Intraassay CV | 4.4 |
| Gene Symbol | IL-1,IL1-BETA,IL1F2 |
| Total Assay Time | 2 hrs 55 mins |
| Sample Volume | Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 1 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 3.9 to 250 pg/mL |
| For Use With (Application) | Colorimetric |
| Test Time | 1 hrs 20 mins |
| Accession Number | P01584 |
| Regulatory Status | RUO |
| Target | IL-1 beta |
| Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| Precision | 1 pg/mL |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3553 |
| Sample Type | Serum,Supernatant |
| Label or Dye | HRP |
Molecular Probes™ CDP-Star™ Substrate (0.25 mM Ready-To-Use)
The CDP-Star™ Substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes.
| Content And Storage | Store the product at 4°C. |
|---|---|
| Format | Bottle |
| Form | Liquid |
| Product Type | ELISA Substrate |
| Substrate Type | Alkaline Phosphatase |
| Product Line | CDP-Star, NovaBright |
Invitrogen™ Tissue Extraction Reagent I
Lysis buffer for total protein extraction from tissue samples
| Protein Family | Cytokines & Receptors |
|---|---|
| Conjugate | HRP |
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | IL6 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
| Assay Sensitivity | 45 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Research Discipline | Immunology |
| Assay Range | 40.96 to 10,000 pg/mL |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hrs 20 mins |
| Detection Method | Colorimetric |
| Assay | Solid-phase sandwich ELISA |
| Accession Number | P26893 |
| Regulatory Status | RUO |
| Target | IL-6 |
| Specificity | The sandwich ELISA antibody pair detects Porcine IL-6. |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 399500 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |