Thermo Scientific MabCaptureC Affinity Matrix
Discover high-performance Protein A chromatography resin designed for efficient and reliable purification of antibodies.
How Can Thermo Scientific™ MabCaptureC™ Affinity Matrix Help You?
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FAQ
It’s a high-flow, agarose-based Protein A–type resin for cost-effective purification of monoclonal IgG. The ligand binds the CH2–CH3 interface of the Fc domain and can also capture a subset of scFv and Fab fragments that contain a VH3 domain.
- Dynamic binding capacity: ≥50 g IgG/L at 10% breakthrough with ~4.8 min residence time.
- Recommended residence time: ≥5 minutes.
- Typical linear velocity: 50–300 cm/h; pressure limit ≤3 bar.
- The pressure-flow curve shows <2 bar pressure drop at 400 cm/h for a 30-cm-ID, 20-cm bed.
- Equilibration/wash: 50 mM Tris, 125 mM NaCl or PBS, pH 7.0–7.5; adding NaCl up to 1.0 M can improve washing.
- Elution: 100 mM citric acid, pH 3.0–3.5.
- Strip: 0.2 M acetic, citric, or phosphoric acid.
- Operating temperature: 2–25 °C.
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