Corning Elplasia 12K Flask

Culture 1000s of spheroids in the vessel footprint of a T-75 flask

Why Choose the Corning™ Elplasia™ 12K Flask?

Ease of Use

The microcavity geometry allows spheroids to remain in place during medium exchange without compromising full recovery at harvest time. The flask’s internal diverter feature allows for minimal disruption of spheroids during liquid handling steps.

Scaffold-Free

The ultra-low attachment (ULA) surface, a proprietary, animal-free, covalently bonded hydrogel, promotes the formation and easy harvesting of spheroids.

Highly Reproducible

The Corning™ Elplasia™ 12K Flask is designed to enable the generation of large quantities of uniform spheroids in a common media reservoir, allowing for equivalent culture conditions for all spheroids.

Applicable Solutions

Spheroid Cell Culture Steps

Gravity, in conjunction with a ULA surface and rounded microcavity geometry, enables the formation of approximately 12,000 spheroids of similar shape and size. The flasks are sterile and ready-to-use. A liquid diverter feature allows for minimal spheroid disruption during liquid handling steps. The microcavity geometry allows spheroids to remain in place during medium exchange steps without compromising full recovery at collection/harvest time.

Discover the Product

1. Seeding Cells

Optimal seeding density for the Corning™ Elplasia™ 12K Flask depends on factors such as cell type, culture duration, and the desired size of spheroids at the time of assessment.

2. Medium Exchange

The microcavities of the Corning™ Elplasia™ 12K Flask are deep enough to permit medium exchange with gentle handling, but not too deep that it is difficult to recover the spheroids when desired. During medium exchange, it is important to maintain the vessel in the incubation position to prevent loss of spheroids. The canted neck and the liquid diverter feature allow for gentle flow of medium into and out of the vessel.

3. Spheroid Collection

Collection volume will depend on the desired final concentration and downstream application. A minimum of 15mL of 1X PBS is recommended for collection; however, growth medium can also be used.

4. In Flask Spheroid Dissociation

Depending on downstream requirements, spheroids can be collected from the Corning™ Elplasia™ 12K Flask as described above for processing, or dissociated into single cells directly in the vessel.