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Invitrogen™ ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System

Product Code. 17133029
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Quantity:
20 Reactions
Unit Size:
20 reactions
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Regulatory: Restrictions may apply to the purchase of this product and we may need to request additional documentation from you

Product Code. 17133029

Brand: Invitrogen™ A11141

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3469.50 EUR valid until 2025-12-19
BEST PRICE on Promo! Use promo code "24110" to get your promotional price.
This item is not returnable. View return policy
Regulatory: Restrictions may apply to the purchase of this product and we may need to request additional documentation from you

Description

The ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit.

The ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen's ViraPower™ HiPerform™ Lentiviral, T-REx™ and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based, regulated (Tetracycline-inducible), high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3/ TO/V5-DEST vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages

  • Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
  • Easy recombination-based cloning using Gateway™ technology
  • Stable, long-term, tetracycline-regulated expression
  • Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems

Key Features

  • WPRE (Woodchuck Posttranscriptional Regulatory Element) from the woodchuck hepatitis virus, increases transgene expression and cPPT (central Polypurine Tract) from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
  • Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene
  • Blasticidin selection marker for stable selection under control of SV40 promoter

Kit includes

  • ViraPower™ HiPerform™ T-Rex™ Gateway™ Vector Kit (Cat. No. A11144)
  • ViraPower™ Bsd Lentiviral Support Kit (K497000)
  • 293FT Cell Line (R70007)
  • Gateway™ LR Clonase™ II Plus Enzyme Mix (12538120)
  • One Shot™ Stbl3™ Chemically Competent E. coli (C737303)
  • Geneticin™ (10131035)
  • pENTR™ Gus positive control plasmid
TRUSTED_SUSTAINABILITY

Specifications

Antibiotic Resistance Bacterial Ampicillin (AmpR), Blasticidin (BsdR)
Content And Storage

ViraPower™ HiPerform™ T-Rex™ Gateway™ Vector Kit (Cat. No. A11144)
• pLenti6.3/TO/V5-DEST vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg
• pLenti3.3/TR vector (40 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 20 μg
• pLenti6.3/TO/V5-GW/lacZ plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg
• Tetracycline (10 mg/mL in water), 1 mL. Store at -20°C, protect from light.
Store at -20°C unless otherwise noted

ViraPower™ Bsd Lentiviral Support Kit (Cat. No. K497000)
• ViraPower™ Lentiviral Packaging Mix (Contains 195 μL of a mixture of pLP1, pLP2, and pLP/VSVG plasmids, at 1 μg/μL in TE Buffer, pH 8.0), 195 μg. Store at -20°C.
• Lipofectamine™ 2000 (Cat. No. 11668027), 0.75 mL. Store at 4°C (Do not freeze)
• Blasticidin powder (Cat. No. R21001), 50 mg. Store at -20°C.

293FT Cell Line (Cat. No. R70007)
• 3 x 106 frozen cells in 1 mL of Freezing Medium, 1 vial. Store in liquid nitrogen

Gateway™ LR Clonase™ II Plus Enzyme Mix (Cat. No. 12538120)
• Gateway™ LR Clonase™ II Plus Enzyme Mix, 40 μL. Store -20°C for up to 6 months, -80°C for long term storage.
• Proteinase K Solution (2 ug/mL), 40 μL

One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)
• Stbl3™ Cells, 6 mL of S.O.C. Medium, 21 x 50 μL. Store at -80°C.

Geneticin™ (Cat. No. 10131035)
• Geneticin™ solution (50 mg/mL), 20 mL, store at 4°C or -20°C
• pENTR™ Gus positive control plasmid, store at -20°C

pUC19 control DNA, (10 pg/μL), 50 μL
Cloning Method Gateway
Constitutive or Inducible System Inducible
Delivery Type Lentiviral
Cell Type 293-FT
Format Kit
Promoter CMV/TO
Vector pLenti
Gateway Enzyme LR Clonase™ II Plus, LR Clonase™ II
For Use With (Equipment) Gateway™, HiPerform™, T-REx™, ViraPower™
For Use With (Application) Protein Expression
Inducing Agent Doxycycline, Tetracycline
Key Functions Viral Expression
No. of Reactions 20 Reactions
PCR Enzyme Proof-Reading Polymerase
Product Line Gateway, HiPerform, T-REx, ViraPower
Product Type Mammalian Expression system
Protein Tag V5 Epitope Tag
Quantity 20 Reactions
Selection Agent (Eukaryotic) Blasticidin
Replication Origin pUC Origin
Selection Marker Eukaryotic BsdR
Selection Marker Promoter PGK Promoter
Special Elements WPRE, cPPT
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For Research Use Only. Not for use in diagnostic procedures.

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